Vibrio fischeri siderophore production drives competitive exclusion during dual-species growth.,Molecular Microbiology

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Vibrio fischeri siderophore production drives competitive exclusion during dual-species growth.
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-04-07 , DOI: 10.1111/mmi.14509
Michaela J Eickhoff ₁ , Bonnie L Bassler _{1,

2}

Affiliation

When two or more bacterial species inhabit a shared niche, often, they must compete for limited nutrients. Iron is an essential nutrient that is especially scarce in the marine environment. Bacteria can use the production, release, and re‐uptake of siderophores, small molecule iron chelators, to scavenge iron. Siderophores provide fitness advantages to species that employ them by enhancing iron acquisition, and moreover, by denying iron to competitors incapable of using the siderophore–iron complex. Here, we show that cell‐free culture fluids from the marine bacterium Vibrio fischeri ES114 prevent the growth of other vibrio species. Mutagenesis reveals the aerobactin siderophore as the inhibitor. Our analysis reveals a gene, that we name aerE, encodes the aerobactin exporter, and LuxT is a transcriptional activator of aerobactin production. In co‐culture, under iron‐limiting conditions, aerobactin production allows V. fischeri ES114 to competitively exclude Vibrio harveyi, which does not possess aerobactin production and uptake genes. In contrast, V. fischeri ES114 mutants incapable of aerobactin production lose in competition with V. harveyi. Introduction of iutA, encoding the aerobactin receptor, together with fhuCDB, encoding the aerobactin importer are sufficient to convert V. harveyi into an “aerobactin cheater.”

中文翻译：

费氏弧菌铁载体的产生导致双物种生长过程中的竞争排斥。

当两种或多种细菌栖息在一个共享的生态位时，它们通常必须竞争有限的营养。铁是一种必需营养素，在海洋环境中尤其稀缺。细菌可以利用铁载体（小分子铁螯合剂）的产生、释放和再摄取来清除铁。铁载体通过增强铁的获取，以及通过拒绝向无法使用铁载体-铁复合物的竞争者提供铁，为利用铁载体的物种提供适应性优势。在这里，我们证明来自海洋细菌费氏弧菌ES114 的无细胞培养液可以阻止其他弧菌物种的生长。诱变揭示了需氧菌素铁载体作为抑制剂。我们的分析揭示了一个基因，我们将其命名为aerE ，编码 aerobactin 输出蛋白，而 LuxT 是 aerobactin 产生的转录激活因子。在共培养中，在铁限制条件下，需氧菌素的产生使得费氏弧菌ES114 能够竞争性地排除不具有需氧菌素产生和摄取基因的哈维氏弧菌。相反，无法产生需氧菌素的费氏弧菌ES114突变体在与哈维弧菌的竞争中失败。引入编码 aerobactin 受体的iutA以及编码 aerobactin 导入器的fhuCDB足以将V. harveyi转化为“aerobactin 作弊者”。

更新日期：2020-04-07

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