当前位置：
X-MOL 学术
›
Bioorgan. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Synthesis of two novel pyrazolo[1,5-a]pyrimidine compounds with antibacterial activity and biophysical insights into their interactions with plasma protein.
Bioorganic Chemistry ( IF 4.5 ) Pub Date : 2020-04-10 , DOI: 10.1016/j.bioorg.2020.103833 Ling-Ling He 1 , Qi Qi 1 , Xin Wang 2 , Yu Li 1 , Yao Zhu 1 , Xiao-Fang Wang 2 , Liang Xu 2
Bioorganic Chemistry ( IF 4.5 ) Pub Date : 2020-04-10 , DOI: 10.1016/j.bioorg.2020.103833 Ling-Ling He 1 , Qi Qi 1 , Xin Wang 2 , Yu Li 1 , Yao Zhu 1 , Xiao-Fang Wang 2 , Liang Xu 2
Affiliation
|
Two novel water-soluble pyrazolo[1,5-a]pyrimidine derivatives, 5-chloro-7-(4-methyl-piperazin -1-yl)-pyrazolo[1,5-a]pyrimidine (CMPS) and N'-(5-chloro-pyrazolo[1,5-a]pyrimidin-7-yl)-N,N-dimethyl -propane-1,3-diamine (NCPS), were synthesized and characterized with antibacterial activity. Then, the interactions of these compounds with bovine serum albumin (BSA) were studied by fluorescence, time-resolved fluorescence, circular dichroism (CD) spectroscopy and molecular docking. The results indicate that both CMPS and NCPS could effectively quench the intrinsic fluorescence of BSA via a static quenching process. The energy transfer from BSA to CMPS and NCPS may occur with high probability. Both CMPS and NCPS bind in the site I of BSA. The hydrophobic force and hydrogen bonds play major roles in the complex formation. Binding constants for both systems show that the affinity of CMPS binding to BSA is stronger than that of NCPS. The results of three-dimensional fluorescence and CD spectra reveal that the binding of CMPS and NCPS to BSA can induce conformational changes of BSA, and the influence of CMPS is slightly stronger than that of NCPS.
中文翻译：
两种具有抗菌活性的吡唑并[1,5-a]嘧啶化合物的合成及其与血浆蛋白相互作用的生物物理学见解。
两种新型水溶性吡唑并[1,5-a]嘧啶衍生物，5-氯-7-（4-甲基哌嗪-1-基）-吡唑并[1,5-a]嘧啶（CMPS）和N'-合成了（5-氯-吡唑并[1,5-a]嘧啶-7-基）-N，N-二甲基丙烷-1,3-二胺（NCPS），并进行了抗菌活性表征。然后，通过荧光，时间分辨荧光，圆二色性（CD）光谱和分子对接研究了这些化合物与牛血清白蛋白（BSA）的相互作用。结果表明，CMPS和NCPS均可通过静态猝灭过程有效猝灭BSA的固有荧光。从BSA到CMPS和NCPS的能量转移可能发生的可能性很高。CMPS和NCPS都绑定在BSA的站点I中。疏水力和氢键在复合物的形成中起主要作用。两种系统的结合常数均表明CMPS结合BSA的亲和力强于NCPS。三维荧光和CD光谱的结果表明，CMPS和NCPS与BSA的结合可以诱导BSA的构象变化，并且CMPS的影响略强于NCPS。
更新日期：2020-04-20
中文翻译：
两种具有抗菌活性的吡唑并[1,5-a]嘧啶化合物的合成及其与血浆蛋白相互作用的生物物理学见解。
两种新型水溶性吡唑并[1,5-a]嘧啶衍生物，5-氯-7-（4-甲基哌嗪-1-基）-吡唑并[1,5-a]嘧啶（CMPS）和N'-合成了（5-氯-吡唑并[1,5-a]嘧啶-7-基）-N，N-二甲基丙烷-1,3-二胺（NCPS），并进行了抗菌活性表征。然后，通过荧光，时间分辨荧光，圆二色性（CD）光谱和分子对接研究了这些化合物与牛血清白蛋白（BSA）的相互作用。结果表明，CMPS和NCPS均可通过静态猝灭过程有效猝灭BSA的固有荧光。从BSA到CMPS和NCPS的能量转移可能发生的可能性很高。CMPS和NCPS都绑定在BSA的站点I中。疏水力和氢键在复合物的形成中起主要作用。两种系统的结合常数均表明CMPS结合BSA的亲和力强于NCPS。三维荧光和CD光谱的结果表明，CMPS和NCPS与BSA的结合可以诱导BSA的构象变化，并且CMPS的影响略强于NCPS。
京公网安备 11010802027423号