GOLDEN2-LIKE (GLK) is a member of the myeloblastosis (MYB) family transcription factor and it plays an important role in the regulation of plastid development and stress tolerance. In this study, a gene named AhGLK1b was identified from a cultivated peanut showing down-regulation in response to low calcium with a complete open reading frame (ORF) of 1212 bp. The AhGLK1b has 99.26% and 96.28% sequence similarities with its orthologs in Arachis ipaensis and A. duranensis, respectively. In the peanut, the AhGLK1b was localized in the nucleus and demonstrated the highest expression in the leaf, followed by the embryo. Furthermore, the expression of AhGLK1b was induced significantly in response to a bacterial pathogen, Ralstonia solanacearum infection. Ectopic expression of AhGLK1b in Arabidopsis showed stronger resistance against important phytopathogenic fungi S. sclerotiorum. It also exhibited high resistance to infection of the bacterial pathogen Pst DC3000. AhGLK1b-expressing Arabidopsis induced defense-related genes including PR10 and Phox/Bem 1 (PBI), which are involved in multiple disease resistance. Taken together, the results suggest that AhGLK1b might be useful in providing dual resistance to fungal and bacterial pathogens as well as tolerance to abiotic stresses.

中文翻译：

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AhGLK1b（GOLDEN2 样转录因子）在拟南芥中的异位表达赋予对真菌和细菌病原体的双重抗性

GOLDEN2-LIKE (GLK) 是成髓细胞增多症 (MYB) 家族转录因子的成员，在调节质体发育和胁迫耐受性中起重要作用。在这项研究中，从栽培花生中鉴定出一个名为 AhGLK1b 的基因，该基因在响应低钙时表现出下调，具有 1212 bp 的完整开放阅读框 (ORF)。AhGLK1b 与其在 Arachis ipaensis 和 A. duranensis 中的直向同源物分别具有 99.26% 和 96.28% 的序列相似性。在花生中，AhGLK1b 定位于细胞核中，在叶中表达最高，其次是胚胎。此外，AhGLK1b 的表达被显着诱导以响应细菌病原体青枯菌感染。AhGLK1b 在拟南芥中的异位表达对重要的植物病原真菌 S. sclerotiorum 表现出更强的抗性。它还表现出对细菌病原体 Pst DC3000 感染的高度抵抗力。表达 AhGLK1b 的拟南芥诱导防御相关基因，包括 PR10 和 Phox/Bem 1 (PBI)，它们参与多种疾病抗性。总之，结果表明 AhGLK1b 可能有助于提供对真菌和细菌病原体的双重抗性以及对非生物胁迫的耐受性。