Emerging investigator series: activated sludge upon antibiotic shock loading: mechanistic description of functional stability and microbial community dynamics,Environmental Science: Water Research & Technology

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Emerging investigator series: activated sludge upon antibiotic shock loading: mechanistic description of functional stability and microbial community dynamics
Environmental Science: Water Research & Technology ( IF 3.5 ) Pub Date : 2020-04-07 , DOI: 10.1039/d0ew00069h
Seungdae Oh _{1,

2,

3,

4} , Donggeon Choi _{1,

2,

3,

4}

Affiliation

Elucidating the functional stability upon environmental stressors is of great practical importance for managing the system performance of various environmental biological processes (e.g., activated sludge). This study quantified the dynamic functional stability upon antibiotic ciprofloxacin (CIP) shock loading using three activated sludge microbial communities developed with different carbon sources, i.e., acetate (A), glucose (G), and starch (S). While the press disturbance (5 mg L⁻¹ CIP shock loading) over two months significantly disrupted the heterotrophic activities of the three communities, the extent of the functional stability (i.e., resistance and resilience) was significantly different. The A and G communities exhibited relatively similar magnitudes of resistance (0.52 and 0.58, respectively) and resilience (0.043 and 0.044, respectively), while the S communities with lower initial species richness and diversity were found to be more sensitive (0.27 and 0.02 for resistance and resilience, respectively) to the antibiotic disturbance. 16S rRNA-gene-based community profiling revealed that the severe disruption to the S communities was associated with a significant reduction of a keystone species population (Novosphingobium) capable of hydrolyzing the glycosidic bonds of starch, i.e., the first and rate-limiting step of the overall community catabolism. Our work highlighted the importance of keystone taxa that disproportionately caused a significant impact on the overall ecosystem's function and stability. These results therefore identifying and monitoring keystone taxa would be useful to help prevent poor performance and catastrophic failure of biological treatment processes, which often occur upon shock loads of toxic chemicals.

中文翻译：

新兴研究者系列：施加抗生素冲击后产生活性污泥：功能稳定性和微生物群落动力学的机理描述

阐明环境压力源的功能稳定性对于管理各种环境生物过程（例如活性污泥）的系统性能具有非常重要的实践意义。这项研究使用三个活性污泥微生物群落开发了定量的抗生素环丙沙星（CIP）冲击负荷，动态活性稳定性由不同的碳源形成，即乙酸（A），葡萄糖（G）和淀粉（S）。尽管两个月的压榨干扰（5 mg L ^-1 CIP冲击负荷）显着破坏了三个群落的异养活动，但功能稳定性的程度（即，抵抗力和弹性）明显不同。A和G群落显示出相对相似的抗性幅度（分别为0.52和0.58）和复原力（分别为0.043和0.044），而具有较低初始物种丰富度和多样性的S群落对它们的敏感性更强（0.27和0.02）。抵抗力和弹性）。的16S rRNA基于基因社区分析显示，严重破坏到S社区与（一个显著减少一个关键物种种群的相关Novosphingobium）能够水解淀粉的糖苷键，即，是整个社区分解代谢的第一步和限速步骤。我们的工作强调了基石分类群的重要性，基石分类群对整个生态系统的功能和稳定性造成了不小的影响。因此，这些结果的识别和监控关键分类单元将有助于防止生物学处理过程的不良性能和灾难性失败，而生物学处理过程通常在有毒化学药品的冲击负荷下发生。

更新日期：2020-04-07

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