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Pentatricopeptide repeat protein PHOTOSYSTEM I BIOGENESIS FACTOR2 is required for splicing of ycf3.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-04-06 , DOI: 10.1111/jipb.12936 Xuemei Wang 1, 2 , Zhipan Yang 1, 2 , Yi Zhang 3 , Wen Zhou 1, 2 , Aihong Zhang 3 , Congming Lu 3
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-04-06 , DOI: 10.1111/jipb.12936 Xuemei Wang 1, 2 , Zhipan Yang 1, 2 , Yi Zhang 3 , Wen Zhou 1, 2 , Aihong Zhang 3 , Congming Lu 3
Affiliation
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To gain a better understanding of the molecular mechanisms of photosystem I (PSI) biogenesis, we characterized the Arabidopsis thaliana photosystem I biogenesis factor 2 (pbf2) mutant, which lacks PSI complex. PBF2 encodes a P‐class pentatricopeptide repeat (PPR) protein. In the pbf2 mutants, we observed a striking decrease in the transcript level of only one gene, the chloroplast gene ycf3, which is essential for PSI assembly. Further analysis of ycf3 transcripts showed that PBF2 is specifically required for the splicing of ycf3 intron 1. Computational prediction of binding sequences and electrophoretic mobility shift assays reveal that PBF2 specifically binds to a sequence in ycf3 intron 1. Moreover, we found that PBF2 interacted with two general factors for group II intron splicing CHLOROPLAST RNA SPLICING2‐ASSOCIATED FACTOR1 (CAF1) and CAF2, and facilitated the association of these two factors with ycf3 intron 1. Our results suggest that PBF2 is specifically required for the splicing of ycf3 intron 1 through cooperating with CAF1 and CAF2. Our results also suggest that additional proteins are required to contribute to the specificity of CAF‐dependent group II intron splicing.
中文翻译：
五聚肽重复蛋白PHOTOSYSTEM I BIOGENESIS FACTOR2是ycf3剪接所必需的。
为了更好地了解光系统I（PSI）生物发生的分子机制，我们对拟南芥光系统I生物发生因子2（pbf2）突变体进行了表征，该突变体缺少PSI复合物。PBF2编码一种P级五肽重复序列（PPR）蛋白。在pbf2突变体中，我们观察到只有一个基因叶绿体基因ycf3的转录水平显着下降，这对于PSI组装是必不可少的。对ycf3转录本的进一步分析表明，PBF2是ycf3剪接的特别要求内含子1。结合序列的计算预测和电泳迁移率变动分析表明，PBF2特异性结合ycf3内含子1中的序列。此外，我们发现PBF2与II组内含子剪接CHLOROPLAST RNA SPLICING2-ASSOCIATED FACTOR1（CAF1）的两个通用因子相互作用）和CAF2，并促进了这两个因素与ycf3内含子1的关联。我们的结果表明，通过与CAF1和CAF2配合使用，对于ycf3内含子1的剪接而言，特别需要PBF2。我们的结果还表明，需要其他蛋白质来促进依赖CAF的II组内含子剪接的特异性。
更新日期：2020-04-06
中文翻译：
五聚肽重复蛋白PHOTOSYSTEM I BIOGENESIS FACTOR2是ycf3剪接所必需的。
为了更好地了解光系统I（PSI）生物发生的分子机制，我们对拟南芥光系统I生物发生因子2（pbf2）突变体进行了表征，该突变体缺少PSI复合物。PBF2编码一种P级五肽重复序列（PPR）蛋白。在pbf2突变体中，我们观察到只有一个基因叶绿体基因ycf3的转录水平显着下降，这对于PSI组装是必不可少的。对ycf3转录本的进一步分析表明，PBF2是ycf3剪接的特别要求内含子1。结合序列的计算预测和电泳迁移率变动分析表明，PBF2特异性结合ycf3内含子1中的序列。此外，我们发现PBF2与II组内含子剪接CHLOROPLAST RNA SPLICING2-ASSOCIATED FACTOR1（CAF1）的两个通用因子相互作用）和CAF2，并促进了这两个因素与ycf3内含子1的关联。我们的结果表明，通过与CAF1和CAF2配合使用，对于ycf3内含子1的剪接而言，特别需要PBF2。我们的结果还表明，需要其他蛋白质来促进依赖CAF的II组内含子剪接的特异性。
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