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Intervention effect of Malus pumila leaf flavonoids on senna-induced acute diarrhea in BALB/c mice.
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2020-04-05 , DOI: 10.1002/fsn3.1549 Ruokun Yi 1, 2, 3 , Yu Tian 4 , Fang Tan 5 , Wenfeng Li 6 , Jianfei Mu 1, 2, 3 , Xingyao Long 1, 2, 3, 7 , Yanni Pan 1, 2, 3, 7 , Xin Zhao 1, 2, 3
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2020-04-05 , DOI: 10.1002/fsn3.1549 Ruokun Yi 1, 2, 3 , Yu Tian 4 , Fang Tan 5 , Wenfeng Li 6 , Jianfei Mu 1, 2, 3 , Xingyao Long 1, 2, 3, 7 , Yanni Pan 1, 2, 3, 7 , Xin Zhao 1, 2, 3
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The Malus pumila leaves are used as a kind of tea drink in China, and there are abundant flavonoids in the leaves of Malus pumila. In this study, BALB/c mice received senna extract solution via gavage, which induced acute diarrhea, and the interventive effect of Malus pumila leaf flavonoids (MPLF) was observed. The results showed that MPLF decreased the diarrhea index, and MPLF also decreased the capillary permeability in the abdominal cavity of mice. The result of serum cytokine detection indicated that MPLF decreased the levels of inflammatory cytokines, including interleukin 6 (IL‐6), interleukin 12 (IL‐12), and tumor necrosis factor alpha (TNF‐α). The results of quantitative polymerase chain reaction (qPCR) indicated that diarrhea decreased the mRNA and protein expression of aquaporin‐3 (AQP3) in the jejunum and aquaporin‐4 (AQP4) in the ileum, which were inhibited by MPLF. By high performance liquid chromatography (HPLC), MPLF mainly contains 10 flavonoids, which are rutin, hyperoside, isoquercitrin, taxifolin, quercitrin, hesperidin, myricetin, baicalin, neohesperidin dihydrochalcone and quercetin, the synergistic effect of these components plays an antidiarrhea role in MPLF. Therefore, MPLF achieved good antidiarrheal effect, which was better than that of the commonly used montmorillonite powder at the same concentration. MPLF is a natural raw material for functional food with an antidiarrheal effect.
中文翻译：
海棠叶黄酮对番泻叶所致BALB/c小鼠急性腹泻的干预作用。
海棠叶在我国被用作茶饮料，海棠叶中含有丰富的黄酮类化合物。本研究通过给BALB/c小鼠灌胃番泻叶提取液引起急性腹泻，观察海棠叶黄酮类化合物（MPLF）的干预作用。结果表明，MPLF降低了小鼠腹泻指数，同时MPLF还降低了小鼠腹腔毛细血管的通透性。血清细胞因子检测结果表明，MPLF降低了炎症细胞因子的水平，包括白细胞介素6（IL-6）、白细胞介素12（IL-12）和肿瘤坏死因子α（TNF-α）。定量聚合酶链反应（qPCR）结果表明，腹泻降低了空肠中水通道蛋白-3（AQP3）和回肠中水通道蛋白-4（AQP4）的mRNA和蛋白表达，而MPLF可抑制这种表达。高效液相色谱（HPLC）检测，MPLF主要含有芦丁、金丝桃苷、异槲皮苷、花旗松素、槲皮甙、橙皮甙、杨梅素、黄芩甙、新橙皮甙二氢查耳酮、槲皮素等10种黄酮类化合物，这些成分的协同作用在MPLF中发挥止泻作用。 。因此，MPLF取得了良好的止泻效果，优于同浓度常用的蒙脱石粉。 MPLF是一种天然的功能性食品原料，具有止泻作用。
更新日期：2020-04-05
中文翻译：
海棠叶黄酮对番泻叶所致BALB/c小鼠急性腹泻的干预作用。
海棠叶在我国被用作茶饮料，海棠叶中含有丰富的黄酮类化合物。本研究通过给BALB/c小鼠灌胃番泻叶提取液引起急性腹泻，观察海棠叶黄酮类化合物（MPLF）的干预作用。结果表明，MPLF降低了小鼠腹泻指数，同时MPLF还降低了小鼠腹腔毛细血管的通透性。血清细胞因子检测结果表明，MPLF降低了炎症细胞因子的水平，包括白细胞介素6（IL-6）、白细胞介素12（IL-12）和肿瘤坏死因子α（TNF-α）。定量聚合酶链反应（qPCR）结果表明，腹泻降低了空肠中水通道蛋白-3（AQP3）和回肠中水通道蛋白-4（AQP4）的mRNA和蛋白表达，而MPLF可抑制这种表达。高效液相色谱（HPLC）检测，MPLF主要含有芦丁、金丝桃苷、异槲皮苷、花旗松素、槲皮甙、橙皮甙、杨梅素、黄芩甙、新橙皮甙二氢查耳酮、槲皮素等10种黄酮类化合物，这些成分的协同作用在MPLF中发挥止泻作用。 。因此，MPLF取得了良好的止泻效果，优于同浓度常用的蒙脱石粉。 MPLF是一种天然的功能性食品原料，具有止泻作用。
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