Lucyoside B is one of the major triterpenoid saponins in the fruit of Luffa cylindrica. Although some chemical and bioactive studies on the extracts of Luffa cylindrica have been reported, pharmacological action of lucyoside B remains obscure. In this study, we explored the anti-inflammatory activity of lucyoside B in activated macrophages. Our data showed that lucyoside B suppressed the proinflammatory mediators, such as iNOS, IL-6 and MCP-1, at the transcriptional and translational levels, as well as the production of NO, indicating the potent anti-inflammatory effect in lipopolysaccharide-primed macrophages. Mechanistically, lucyoside B inhibited the phosphorylation and degradation of IκBα and thus prevented the nuclear translocation of p65 to suppress NF-κB transcriptional activity. Concurrently, it also decreased JNK1/2, ERK1/2 and p38 phosphorylation to reduce the transcriptional activity of AP-1. Our study suggests that lucyoside B may be a beneficial food constituent for inflammatory diseases.

Lucyoside B是Luffa cylindrica果实中主要的三萜皂甙之一。尽管对丝瓜提取物进行了一些化学和生物活性研究已有报道，lucyoside B的药理作用仍然不清楚。在这项研究中，我们探讨了活化的巨噬细胞中葡糖苷B的抗炎活性。我们的数据表明，葡糖苷B在转录和翻译水平以及NO的产生中抑制了促炎性介质，例如iNOS，IL-6和MCP-1，表明在脂多糖引发的巨噬细胞中具有有效的抗炎作用。 。从机理上讲，葡糖苷B抑制了IκBα的磷酸化和降解，从而阻止了p65的核易位，从而抑制了NF-κB的转录活性。同时，它也减少了JNK1 / 2，ERK1 / 2和p38的磷酸化，从而降低了AP-1的转录活性。我们的研究表明，葡糖苷B可能是炎症性疾病的有益食品。