(R)-2-chloro-1-(2, 4-dichlorophenyl) ethanol ((R)-CPEO) is an important chiral intermediate for antifungal drug synthesis. (R)-CPEO can be produced from 2-chloro-1-(2, 4-dichlorophenyl) ethanone (CPE) via a mutant short-chain dehydrogenase/reductase from Novosphingobium aromaticivorans (NaSDR). The Vmax of a mutant NaSDR-G145A/I199L toward CPE (6.32 U mg⁻¹) was greater than that of wild-type NaSDR (2.58 U mg⁻). The Km of mutant NaSDR-G145A/I199L toward CPE (0.23 mM) was less than that of wild-type NaSDR (0.38 mM), indicating that the substrate affinity of mutant NaSDR-G145A/I199L was the greater of the two. Docking simulations were used to illustrate the mechanisms of the increased enzyme activity of NaSDR-G145A/I199L; these showed that NaSDR-G145A/I199L presented a more effective docking posture than that of the wild-type enzyme. Further, NaSDR-G145A/I199L and glucose dehydrogenase (GDH) were used to transform 120 g/L CPE into (R)-CPEO. After 6 h, the conversion rate and enantiomeric excess values were 99% and 99.95%, respectively. The present study provides a practical method for high substrate loading of (R)-CPEO for industrial-scale applications.

（R）-2-氯-1-（2,4-二氯苯基）乙醇（（R）-CPEO）是抗真菌药物合成的重要手性中间体。（R）-CPEO可以由2-氯-1-（2，4-二氯苯基）乙酮（CPE）经由芳族新孢子虫（Novosphingobium aromaivorans）的突变短链脱氢酶/还原酶（Na SDR）制得。所述的Vmax的突变体的钠SDR-G145A / I199L朝向CPE（6.32û毫克^-1）比野生型更大的Na SDR（2.58û毫克^- ）。Na SDR-G145A / I199L突变体对CPE的Km（0.23 mM）小于野生型NaSDR（0.38 mM），表明突变型Na SDR-G145A / I199L的底物亲和力是两者中较大的。对接模拟用于说明Na SDR-G145A / I199L酶活性增加的机制。这些表明Na SDR-G145A / I199L呈现出比野生型酶更有效的对接姿势。此外，使用Na SDR-G145A / I199L和葡萄糖脱氢酶（GDH）将120g / L CPE转化为（R）-CPEO。6小时后，转化率和对映体过量值分别为99％和99.95％。本研究为工业规模应用的（R）-CPEO的高底物负载提供了一种实用的方法。