Noninvasive In Situ Ratiometric Imaging of Biometals Based on Self-Assembled Peptide Nanoribbon.,Analytical Chemistry

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Noninvasive In Situ Ratiometric Imaging of Biometals Based on Self-Assembled Peptide Nanoribbon.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-04-02 , DOI: 10.1021/acs.analchem.9b05490
Li Lei ₁ , Min Li ₁ , Sufen Wu ₁ , Zhiai Xu ₁ , Ping Geng ₁ , Yang Tian ₁ , Ying Fu ₂ , Wen Zhang ₃

Affiliation

Development of probes for accurate sensing and imaging of biometals in situ is still a growing interest owing to their crucial roles in cellular metabolism, neurotransmission, and apoptosis. Among them, Zn²⁺ and Cu²⁺ are two important cooperative biometals closely related to Alzheimer’s disease (AD). Herein, we developed a multifunctional probe based on self-assembling peptide nanoribbon for ratiometric sensing of Zn²⁺, Cu²⁺, or Zn²⁺ and Cu²⁺ simultaneously. Uniform peptide nanoribbon (AQZ@NR) was rationally designed by coassembling a Zn²⁺-specific ligand AQZ-modified peptide (AQZKL-7) with peptide KL-7. The nanoribbon further combined with Cu²⁺-sensitive near-infrared quantum dots (NIR QDs) and Alexa Fluor 633 as an inner reference molecule, which was endowed with the capability for ratiometric Zn²⁺ and Cu²⁺ imaging at the same time. The peptide-based probe exhibited good specificity to Zn²⁺ and Cu²⁺ without interference from other ions. Importantly, the nanoprobe was successfully applied for noninvasive Zn²⁺ and Cu²⁺ monitoring in both living cells and zebrafish via multicolor fluorescence imaging. This gives insights into the dynamic Zn²⁺ and Cu²⁺ distribution in an intracellular and in vivo mode, as well as understanding the neurotoxicity of high concentration of Zn²⁺ and Cu²⁺. Therefore, the self-assembled nanoprobe shows great promise in multiplexed detection of many other biometals and biomolecules, which will benefit the diagnosis and treatment of AD in clinical applications.

中文翻译：

基于自组装肽纳米带的生物金属非侵入性原位比例成像。

由于其在细胞代谢，神经传递和细胞凋亡中的关键作用，开发用于精确地原位感测和成像生物金属的探针仍引起越来越多的兴趣。其中，Zn ²⁺和Cu ²⁺是与阿尔茨海默氏病（AD）密切相关的两种重要的协同生物金属。在本文中，我们开发了一种基于自组装肽纳米带的多功能探针，用于同时感测Zn 2 ⁺，Cu ²⁺或Zn ²⁺和Cu ²⁺。通过将Zn ²⁺特异性配体AQZ修饰的肽（AQZKL-7）与肽KL-7共组装，合理设计了均匀的肽纳米带（AQZ @ NR）。纳米带进一步与铜结合²⁺敏感的近红外量子点（NIR QDs）和Alexa Fluor 633作为内部参考分子，具有同时进行Zn ²⁺和Cu ²⁺比例成像的能力。基于肽的探针对Zn ²⁺和Cu ²⁺表现出良好的特异性，而不受其他离子的干扰。重要的是，纳米探针已通过多色荧光成像技术成功应用于活细胞和斑马鱼的无创Zn ²⁺和Cu ²⁺监测。这提供了对动态Zn ²⁺和Cu ^2+的洞察力分布在细胞内和体内模式，以及了解高浓度的Zn ²⁺和Cu ²⁺的神经毒性。因此，自组装的纳米探针在多种其他生物金属和生物分子的多重检测中显示出广阔的前景，这将有助于临床应用中AD的诊断和治疗。

更新日期：2020-04-02

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