Production of Methionol from 3-Methylthiopropionaldehyde by Catalysis of the Yeast Alcohol Dehydrogenase Adh4p.,Journal of Agricultural and Food Chemistry

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Production of Methionol from 3-Methylthiopropionaldehyde by Catalysis of the Yeast Alcohol Dehydrogenase Adh4p.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-04-09 , DOI: 10.1021/acs.jafc.0c00776
Yixin Che _{1,

2,

3} , Sheng Yin _{1,

2,

3} , Hui Wang _{1,

2,

3} , Huaqing Yang _{1,

2,

3} , Ruixin Xu _{1,

2,

3} , Qi Wang _{1,

2,

3} , Yiping Wu _{1,

2,

3} , Julien Boutet _{4,

5} , Robert Huet _{4,

5} , Chengtao Wang _{1,

2,

3}

Affiliation

Methionol is a sulfur-containing aroma compound that contributes to the flavors of fermented foods. In this work, a novel method for methionol production was established using 3-methylthiopropionaldehyde (MMP) and alcohol dehydrogenase (ADH). First, expression of seven ADH genes was analyzed in yeast fermentation added with MMP. Only ADH4 displayed an evident increased expression in response to MMP. ADH4 was then overexpressed in Saccharomyces cerevisiae S288c and Escherichia coli BL21. The recombinant yeast strain S4 produced more methionol than control strain in MMP fermentation. Furthermore, 0.55 g/L 42 kDa Adh4p was prepared from E. coli by induced expression and purification. A fed-batch catalysis system was finally established to produce methionol from MMP by Adh4p. In 10 h of continuous catalysis, the conversion rate of MMP remained 80-95%, and a final yield of 0.2 g/L methionol was achieved. This work proposed a novel method for methionol production by enzymatic catalysis with a potential application prospect in industry.

中文翻译：

通过酵母乙醇脱氢酶Adh4p的催化，由3-甲基硫代丙醛生产甲硫酚。

甲硫醇是一种含硫的芳香化合物，有助于发酵食品的风味。在这项工作中，使用3-甲基硫代丙醛（MMP）和醇脱氢酶（ADH）建立了一种生产甲硫酚的新方法。首先，在添加了MMP的酵母发酵中分析了七个ADH基因的表达。只有ADH4响应MMP表现出明显增加的表达。然后ADH4在酿酒酵母S288c和大肠杆菌BL21中过表达。在MMP发酵中，重组酵母菌株S4产生的甲硫酚比对照菌株多。此外，通过诱导表达和纯化从大肠杆菌制备了0.55g / L 42kDa Adh4p。最终建立了分批补料催化系统，以通过Adh4p从MMP生产甲硫醇。在连续催化10小时后，MMP的转化率保持在80-95％，最终产量为0.2 g / L甲硫醇。这项工作提出了一种酶催化生产甲硫酚的新方法，在工业上具有潜在的应用前景。

更新日期：2020-04-24

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