Although inflammation is a host defense mechanism, chronic inflammation mediates several diseases, including cancer, allergy, asthma, and autoimmune diseases, and reportedly, it is associated with a 60% mortality rate. There are several reports on the anti-inflammatory effects of Curcuma longa and Allium hookeri. However, although they can be used as culinary materials and have biological effects, they are not effective anti-inflammatory agents. In this study, we evaluated the synergic effect of C. longa and A. hookeri in order to confirm the possibility of a new anti-inflammatory agent. Based on cell viability and cytokine analyses, the appropriate ratio of C. longa and A. hookeri was confirmed using an air pouch animal model. Then, the anti-inflammatory effect of C. longa and A. hookeri co-treatment was evaluated by measuring the immune cell count and cytokines in the exudate and by comparing the morphological changes and cytokines in inflamed skin samples. Additionally, we evaluated the NF-κB/COX-2 pathway and iNOS levels. The active constituents detected in C. longa were demethoxycurcumin and bisdemethoxycurcumin, and that detected in A. hookeri was methylsulfonylmethane. An in vitro assessment determined the appropriate drug ratio as 3:7. In a carrageenan-induced inflammatory model, co-treatment effectively suppressed inflammatory cytokines, including IFN-γ, IL-1β, IL-6, IL-13, and IL-17, and recovered inflammation-related morphological changes in the skin. The anti-inflammatory effect of the co-treatment was mediated through the NF-κB/COX-2 pathway and iNOS inhibition. We concluded that co-treatment with C. longa and A. hookeri synergistically inhibited inflammation via the NF-κB/COX-2/iNOS pathway.

尽管炎症是宿主防御机制，但慢性炎症会介导多种疾病，包括癌症，过敏，哮喘和自身免疫性疾病，据报道，其死亡率高达60％。关于姜黄和葱属的抗炎作用的报道已有几篇。然而，尽管它们可以用作烹饪材料并具有生物学作用，但是它们不是有效的抗炎剂。在这项研究中，我们评估了C. longa和A. hookeri的协同作用，以确认新型抗炎药的可能性。根据细胞生存力和细胞因子分析，适当比例的长假丝酵母和A. hookeri使用气袋动物模型确认。然后，通过测量渗出液中的免疫细胞计数和细胞因子，并比较发炎的皮肤样本中的形态变化和细胞因子，来评估长假丝酵母和钩形假单胞菌共同治疗的抗炎作用。此外，我们评估了NF-κB/ COX-2途径和iNOS水平。Long C. longa中检测到的活性成分是脱甲氧基姜黄素和双脱甲氧基姜黄素，而A. hookeri中检测到的活性成分是甲基磺酰甲烷。一种在体外评估确定合适的药物比例为3：7。在角叉菜胶诱导的炎症模型中，共同治疗可有效抑制炎症细胞因子，包括IFN-γ，IL-1β，IL-6，IL-13和IL-17，并恢复皮肤中与炎症相关的形态学变化。联合治疗的抗炎作用是通过NF-κB/ COX-2途径和iNOS抑制作用介导的。我们得出的结论是，与C. longa和A. hookeri共同治疗可通过NF-κB/ COX-2 / iNOS途径协同抑制炎症。