Scientific Reports ( IF 3.8 ) Pub Date : 2020-03-30 , DOI: 10.1038/s41598-020-62195-5 Diptaraj S Chaudhari 1, 2 , Dhiraj P Dhotre 1 , Dhiraj M Agarwal 3 , Akshay H Gaike 1, 3 , Devika Bhalerao 3 , Parmeshwar Jadhav 3 , Dattatray Mongad 1 , Himangi Lubree 3 , Vilas P Sinkar 1 , Ulhas K Patil 2, 4 , Sundeep Salvi 5 , Ashish Bavdekar 6 , Sanjay K Juvekar 3 , Yogesh S Shouche 1
The human microbiome plays a key role in maintaining host homeostasis and is influenced by age, geography, diet, and other factors. Traditionally, India has an established convention of extended family arrangements wherein three or more generations, bound by genetic relatedness, stay in the same household. In the present study, we have utilized this unique family arrangement to understand the association of age with the microbiome. We characterized stool, oral and skin microbiome of 54 healthy individuals from six joint families by 16S rRNA gene-based metagenomics. In total, 69 (1.03%), 293 (2.68%) and 190 (8.66%) differentially abundant OTUs were detected across three generations in the gut, skin and oral microbiome, respectively. Age-associated changes in the gut and oral microbiome of patrilineal families showed positive correlations in the abundance of phyla Proteobacteria and Fusobacteria, respectively. Genera Treponema and Fusobacterium showed a positive correlation with age while Granulicatella and Streptococcus showed a negative correlation with age in the oral microbiome. Members of genus Prevotella illustrated high abundance and prevalence as a core OTUs in the gut and oral microbiome. In conclusion, this study highlights that precise and perceptible association of age with microbiome can be drawn when other causal factors are kept constant.
中文翻译:
印度父系家庭的肠道,口腔和皮肤微生物组显示出与年龄的明显关联。
人类微生物组在维持宿主体内稳态中起着关键作用,并受年龄,地理,饮食和其他因素的影响。传统上,印度有大家庭安排的惯例,其中三代或三代以上受遗传关系约束的人住在同一家庭。在本研究中,我们利用这种独特的家庭安排来了解年龄与微生物组的关联。我们通过基于16S rRNA基因的宏基因组学对来自六个关节家族的54名健康个体的粪便,口腔和皮肤微生物组进行了特征分析。总共在肠道,皮肤和口腔微生物组的三代中分别检测到69(1.03%),293(2.68%)和190(8.66%)差异丰富的OTU。父系家庭的肠道和口腔微生物组的年龄相关变化分别显示了门菌群和变形杆菌的正相关。属梅毒螺旋体和梭形杆菌表现出与年龄呈正相关关系,而Granulicatella和链球菌显示,在口腔微生物与年龄呈负相关。Prevotella属的成员显示出很高的丰度和患病率,是肠道和口腔微生物组中的核心OTU。总而言之,这项研究强调了在其他因果关系保持不变的情况下,可以得出年龄与微生物组的精确且可感知的关联。