Intracellular Wireless Analysis of Single Cells by Bipolar Electrochemiluminescence Confined in a Nanopipette.,Angewandte Chemie International Edition

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Intracellular Wireless Analysis of Single Cells by Bipolar Electrochemiluminescence Confined in a Nanopipette.
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2020-03-25 , DOI: 10.1002/anie.202002323
Yuling Wang ₁ , Rong Jin ₁ , Neso Sojic ₂ , Dechen Jiang ₁ , Hong-Yuan Chen ₁

Affiliation

The inside walls of a nanopipette tip are decorated by a Pt deposit that is used as an open bipolar electrochemiluminescence (ECL) device to achieve intracellular wireless electroanalysis. The synergetic actions of nanopipette and of bipolar ECL lead to the spatial confinement of the voltage drop at the level of the Pt deposit, which generates ECL emission from luminol. The porous structure of Pt deposit permits the electrochemical transport of intracellular molecules into the nanopipette that is coupled with enzymatic reactions. Thus, the intracellular concentrations of hydrogen peroxide or glucose are measured in vivo as well as the intracellular sphingomyelinase activity. In comparison with the classic bipolar ECL, the remarkably low potential applied in our approach is restricted inside the nanopipette and it minimizes the potential bias of the voltage on the cellular activity. Accordingly, this wireless ECL approach provides a new direction for analysis of single living cells.

中文翻译：

通过限制在纳米移液管中的双极电化学发光对单个细胞进行细胞内无线分析。

纳米移液器吸头的内壁由Pt沉积物装饰，Pt沉积物用作开放式双极电化学发光（ECL）设备以实现细胞内无线电分析。纳米吸管和双极ECL的协同作用导致电压降在Pt沉积层的空间限制，从而从鲁米诺产生ECL发射。Pt沉积物的多孔结构允许细胞内分子通过电化学方式转移到纳米吸管中。因此，在体内测量细胞内过氧化氢或葡萄糖的浓度以及细胞内鞘磷脂酶活性。与经典的双极ECL相比，在我们的方法中施加的极低电势被限制在纳米移液器内部，并且它使电压对细胞活性的电势偏置最小化。因此，这种无线ECL方法为分析单个活细胞提供了新的方向。

更新日期：2020-03-25

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