The complement system may be activated in the posterior segment of the eye with chorioretinal disease, which may be reflected to the concentration of anaphylatoxins in the aqueous humor. Little is known about the distribution of anaphylatoxins in the aqueous and vitreous humor. The aim of the present study was to investigate the distribution of anaphylatoxin concentration in the aqueous and vitreous humor of the eyes with idiopathic epiretinal membrane or idiopathic macular hole. This was an experimental, observational case series. This study included 43 eyes from 43 patients; 29 eyes with idiopathic epiretinal membrane, and 14 eyes with idiopathic macular hole. All 43 eyes underwent cataract surgery and vitrectomy. The aqueous and vitreous humor were collected at the surgery. The anaphylatoxin concentrations were measured by using a cytometric beads array, and the respective C3a, C4a, and C5a concentrations were 2.003 ± 0.679 (mean ± standard deviation) ng/ml, 1.389 ± 0.419 ng/ml, and 0.003 ± 0.004 ng/ml in the aqueous humor, and 1.236 ± 0.642 ng/ml, 1.250 ± 0.542 ng/ml, and 0.048 ± 0.069 ng/ml in the vitreous humor. The mean C3a concentration in the aqueous humor was significantly higher than in the vitreous humor in 43 eyes of iMH and iERM (P < 0.001). The mean C4a concentration showed no significant difference between the aqueous humor and vitreous humor (P = 0.282), and the mean C5a in the aqueous humor was significantly lower than in the vitreous humor overall (P < 0.001). The C3a concentration in the aqueous humor strongly correlated with that in the vitreous humor (R = 0.510, P < 0.001). The concentrations of C4a and C5a in the aqueous humor moderately correlated with those in the vitreous humor (C4a; R = 0.356, P = 0.019, C5a; R = 0.464, P = 0.022). In conclusion, the anaphylatoxin concentrations measured by cytometric beads array in the aqueous humor may be associated with those measured in the vitreous humor.

中文翻译：

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玻璃体视网膜界面异常的眼睛中房水和玻璃体液中的过敏毒素浓度。

补体系统可能在脉络膜视网膜疾病的眼后段被激活，这可能反映为房水中过敏毒素的浓度。关于过敏毒素在房水和玻璃体液中的分布知之甚少。本研究的目的是研究特发性视网膜前膜或特发性黄斑裂孔中眼房水和玻璃体液中过敏毒素浓度的分布。这是一个实验性，观察性的案例系列。这项研究包括来自43位患者的43只眼睛；特发性视网膜前膜29眼，特发性黄斑裂孔14眼。所有的43只眼睛都进行了白内障手术和玻璃体切除术。在手术时收集房水和玻璃体液。使用细胞计数珠阵列测量过敏毒素的浓度，相应的C3a，C4a和C5a浓度分别为2.003±0.679（平均值±标准偏差）ng / ml，1.389±0.419 ng / ml和0.003±0.004 ng / ml在房水中的浓度为1.236±0.642 ng / ml，在玻璃体液中为1.250±0.542 ng / ml和0.048±0.069 ng / ml。在iMH和iERM的43只眼中，房水中的C3a平均浓度显着高于玻璃体液（P <0.001）。C4a的平均浓度在房水和玻璃体液之间没有显着差异（P = 0.282），房水的平均C5a明显低于玻璃体液的总体水平（P <0.001）。房水中的C3a浓度与玻璃体液中的C3a浓度高度相关（R = 0.510，P <0.001）。房水中的C4a和C5a浓度与玻璃体液中的浓度适度相关（C4a； R = 0.356，P = 0.019，C5a； R = 0.464，P = 0.022）。总之，房水中房水中通过细胞珠阵列测定的过敏毒素浓度可能与玻璃体液中测定的浓度有关。