当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Photocontrolled Thermosensitive Electrochemiluminescence Hydrogel for Isocarbophos Detection.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-04-03 , DOI: 10.1021/acs.analchem.0c00719 Jiahui Sun 1 , Fu Zhou 1 , Hui Hu 1 , Na Li 1 , Mengmeng Xia 1 , Li Wang 1 , Xiayan Wang 2 , Guangfeng Wang 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-04-03 , DOI: 10.1021/acs.analchem.0c00719 Jiahui Sun 1 , Fu Zhou 1 , Hui Hu 1 , Na Li 1 , Mengmeng Xia 1 , Li Wang 1 , Xiayan Wang 2 , Guangfeng Wang 1
Affiliation
|
Endowing specificity and controllability with the electrochemiluminescence (ECL) thermosensitive hydrogels is vitally crucial to expanding their sensing applications. Herein, a novel photocontrolled thermosensitive electrochemiluminescence hydrogel (PT-ECL hydrogel) sensing platform with sufficient simplicity, specificity, and precise controllability is proposed, for the first time, by the integration of Ru(bpy)32+ (bpy = 2,2'-bipyridine) derivatives (signal reporter), split aptamers (recognition unites), and Au nanorods (AuNRs) (photothermal energy converter) into the poly(N-isopropylacrylamide) (pNIPAM) matrix. In the presence of the model target isocarbophos (ICP), the conjugation of two split aptamers initiated the ECL-resonance energy transfer (ECL-RET) between the Au nanorods and the Ru(bpy)32+ centers. Surprisingly, under the irradiation of near-infrared (NIR) light, the photothermal effect of AuNRs prompted the shrinkage of the hydrogel, resulting in the enhancement of the ECL-RET and further ∼7 times signal amplification. Consequently, the PT-ECL hydrogel sensing platform performed well for ICP detection with a low detection limit of 20 pM (S/N = 3) and a wide linear range from 50 pM to 4 μM, with great stability and repeatability. Obviously, the results showed that AuNRs utilized in this study served the role as not only the ECL-RET acceptor but also the photothermal converter to prompt the phase change of the PT-ECL hydrogel precisely and simply controlled by NIR light. Use of the proposed PT-ECL hydrogel detection scheme is a first step toward enabling a newly upgraded highly sensitive and selective hydrogel-based assay and also paving the way for the application of smart photothermal reagents.
中文翻译：
光控制的热敏电化学发光水凝胶用于异卡波菲的检测。
赋予电化学发光（ECL）热敏水凝胶特异性和可控性对于扩大其传感应用至关重要。本文首次通过整合Ru（bpy）32+（bpy = 2,2'）首次提出了一种具有足够的简便性，特异性和精确可控性的新型光控热敏电化学发光水凝胶（PT-ECL水凝胶）传感平台。 -联吡啶）衍生物（信号报告子），分裂适体（识别单元）和金纳米棒（AuNRs）（光热能转换器）进入聚（N-异丙基丙烯酰胺）（pNIPAM）基质。在存在模型目标异卡波菲（ICP）的情况下，两个分裂的适体的缀合引发了Au纳米棒和Ru（bpy）32+中心之间的ECL共振能量转移（ECL-RET）。出奇，在近红外光的照射下，AuNRs的光热效应促使水凝胶收缩，导致ECL-RET增强，信号放大约7倍。因此，PT-ECL水凝胶传感平台以20 pM的低检测限（S / N = 3）和从50 pM到4μM的宽线性范围在ICP检测中表现出色，具有出色的稳定性和可重复性。显然，结果表明，在这项研究中使用的AuNRs不仅充当ECL-RET受体，而且还充当光热转换器，以促进PT-ECL水凝胶的相变精确而简单地由NIR光控制。
更新日期：2020-04-23
中文翻译：
光控制的热敏电化学发光水凝胶用于异卡波菲的检测。
赋予电化学发光（ECL）热敏水凝胶特异性和可控性对于扩大其传感应用至关重要。本文首次通过整合Ru（bpy）32+（bpy = 2,2'）首次提出了一种具有足够的简便性，特异性和精确可控性的新型光控热敏电化学发光水凝胶（PT-ECL水凝胶）传感平台。 -联吡啶）衍生物（信号报告子），分裂适体（识别单元）和金纳米棒（AuNRs）（光热能转换器）进入聚（N-异丙基丙烯酰胺）（pNIPAM）基质。在存在模型目标异卡波菲（ICP）的情况下，两个分裂的适体的缀合引发了Au纳米棒和Ru（bpy）32+中心之间的ECL共振能量转移（ECL-RET）。出奇，在近红外光的照射下，AuNRs的光热效应促使水凝胶收缩，导致ECL-RET增强，信号放大约7倍。因此，PT-ECL水凝胶传感平台以20 pM的低检测限（S / N = 3）和从50 pM到4μM的宽线性范围在ICP检测中表现出色，具有出色的稳定性和可重复性。显然，结果表明，在这项研究中使用的AuNRs不仅充当ECL-RET受体，而且还充当光热转换器，以促进PT-ECL水凝胶的相变精确而简单地由NIR光控制。
京公网安备 11010802027423号