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Reduction of Phakopsora pachyrhizi infection on soybean through host- and spray-induced gene silencing.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-03-20 , DOI: 10.1111/mpp.12931 Dongfang Hu 1 , Zhi-Yuan Chen 1 , Chunquan Zhang 2 , Mala Ganiger 1
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-03-20 , DOI: 10.1111/mpp.12931 Dongfang Hu 1 , Zhi-Yuan Chen 1 , Chunquan Zhang 2 , Mala Ganiger 1
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Asian soybean rust (ASR), caused by the obligate fungal pathogen Phakopsora pachyrhizi, often leads to significant yield losses and can only be managed through fungicide applications currently. In the present study, eight urediniospore germination or appressorium formation induced P. pachyrhizi genes were investigated for their feasibility to suppress ASR through a bean pod mottle virus (BPMV)‐based host‐induced gene silencing (HIGS) strategy. Soybean plants expressing three of these modified BPMV vectors suppressed the expression of their corresponding target gene by 45%–80%, fungal biomass accumulation by 58%–80%, and significantly reduced ASR symptom development in soybean leaves after the plants were inoculated with P. pachyrhizi, demonstrating that HIGS can be used to manage ASR. In addition, when the in vitro synthesized double‐stranded RNAs (dsRNAs) for three of the genes encoding an acetyl‐CoA acyltransferase, a 40S ribosomal protein S16, and glycine cleavage system H protein were sprayed directly onto detached soybean leaves prior to P. pachyrhizi inoculation, they also resulted in an average of over 73% reduction of pustule numbers and 75% reduction in P. pachyrhizi biomass accumulation on the detached leaves compared to the controls. To the best of our knowledge, this is the first report of suppressing P. pachyrhizi infection in soybean through both HIGS and spray‐induced gene silencing. It was demonstrated that either HIGS constructs targeting P. pachyrhizi genes or direct dsRNA spray application could be an effective strategy for reducing ASR development on soybean.
中文翻译：
通过宿主和喷雾诱导的基因沉默减少大豆豆薯层锈菌感染。
亚洲大豆锈病 (ASR) 由专性真菌病原菌豆薯层锈菌(Phakopsora pachyrhizi)引起，通常会导致严重的产量损失，目前只能通过施用杀菌剂来控制。在本研究中，研究了八个诱导豆薯根孢子萌发或附着胞形成的基因通过基于豆荚斑驳病毒（BPMV）的宿主诱导基因沉默（HIGS）策略抑制ASR的可行性。表达三种改良 BPMV 载体的大豆植物在接种PMV后，将相应靶基因的表达抑制了 45%–80%，真菌生物量积累了 58%–80%，并显着减少了大豆叶片 ASR 症状的发生。 . pachyrhizi ，证明 HIGS 可用于管理 ASR。此外，当在体外合成编码乙酰辅酶A酰基转移酶、40S核糖体蛋白S16和甘氨酸裂解系统H蛋白的三个基因的双链RNA（dsRNA）时，在P.与对照相比，豆薯接种后，脓疱数量平均减少 73% 以上，离体叶片上豆薯生物量积累平均减少 75%。据我们所知，这是第一份通过 HIGS 和喷雾诱导的基因沉默抑制大豆中豆薯赤霉感染的报道。结果表明，针对豆薯基因的 HIGS 构建体或直接 dsRNA 喷雾应用可能是减少大豆 ASR 发展的有效策略。
更新日期：2020-03-20
中文翻译：
通过宿主和喷雾诱导的基因沉默减少大豆豆薯层锈菌感染。
亚洲大豆锈病 (ASR) 由专性真菌病原菌豆薯层锈菌(Phakopsora pachyrhizi)引起，通常会导致严重的产量损失，目前只能通过施用杀菌剂来控制。在本研究中，研究了八个诱导豆薯根孢子萌发或附着胞形成的基因通过基于豆荚斑驳病毒（BPMV）的宿主诱导基因沉默（HIGS）策略抑制ASR的可行性。表达三种改良 BPMV 载体的大豆植物在接种PMV后，将相应靶基因的表达抑制了 45%–80%，真菌生物量积累了 58%–80%，并显着减少了大豆叶片 ASR 症状的发生。 . pachyrhizi ，证明 HIGS 可用于管理 ASR。此外，当在体外合成编码乙酰辅酶A酰基转移酶、40S核糖体蛋白S16和甘氨酸裂解系统H蛋白的三个基因的双链RNA（dsRNA）时，在P.与对照相比，豆薯接种后，脓疱数量平均减少 73% 以上，离体叶片上豆薯生物量积累平均减少 75%。据我们所知，这是第一份通过 HIGS 和喷雾诱导的基因沉默抑制大豆中豆薯赤霉感染的报道。结果表明，针对豆薯基因的 HIGS 构建体或直接 dsRNA 喷雾应用可能是减少大豆 ASR 发展的有效策略。
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