Light-sheet microscopy has been widely used in high-speed fluorescence imaging with low phototoxicity, while the trade-off between the field-of-view and optical sectioning capability limits its application in large-scale imaging. Although Bessel beam light-sheet microscopy greatly enhances the light-sheet length with the self-healing ability, it suffers from the strong side-lobe effect. To solve these problems, we introduce the photobleaching imprinting technique in Bessel beam light-sheet microscopy. By extracting the non-linear photobleaching-induced fluorescence decay, we get rid of the large concentric side lobe structures of the Bessel beam to achieve uniform isotropic resolution across a large field-of-view for large-scale fluorescence imaging. Both numerical simulations and experimental results on various samples are demonstrated to show our enhanced resolution and contrast over traditional Bessel-beam light-sheet microscopy.

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通过光漂白压印提高贝塞尔光束光片荧光显微镜的轴向分辨率

光片显微镜已被广泛用于具有低光毒性的高速荧光成像，而视场和光学切片能力之间的权衡限制了其在大规模成像中的应用。尽管Bessel光束的光片显微镜可以通过自愈功能极大地增加光片的长度，但是它具有较强的旁瓣效应。为了解决这些问题，我们在贝塞尔光束光片显微镜中引入了光漂白压印技术。通过提取非线性光致漂白引起的荧光衰减，我们摆脱了贝塞尔光束的大同心旁瓣结构，从而在大型视场中实现了大范围的均匀等向分辨率，从而进行了大规模的荧光成像。