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In Situ Formation of Gold Nanoparticles Decorated Ti3C2 MXenes Nanoprobe for Highly Sensitive Electrogenerated Chemiluminescence Detection of Exosomes and Their Surface Proteins.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-24 , DOI: 10.1021/acs.analchem.0c00469 Huixin Zhang 1, 2 , Zonghua Wang 2 , Feng Wang 1 , Yimeng Zhang 1 , Hongye Wang 1 , Yang Liu 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-03-24 , DOI: 10.1021/acs.analchem.0c00469 Huixin Zhang 1, 2 , Zonghua Wang 2 , Feng Wang 1 , Yimeng Zhang 1 , Hongye Wang 1 , Yang Liu 1
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In this work, an ultrasensitive electrogenerated chemiluminescence (ECL) biosensor for exosomes and their surface proteins was developed by the in situ formation of gold nanoparticles (AuNPs) decorated Ti3C2 MXenes hybrid with aptamer modification (AuNPs-MXenes-Apt). In this strategy, the exosomes were efficiently captured on an exosome recognized CD63 aptamer modified electrode interface. Meanwhile, in situ formation of gold nanoparticles on single layer Ti3C2MXenes with aptamer (MXenes-Apt) modification was obtained, in which MXenes acted as both reductants and stabilizer, and no additional reductant and stabilizer involved. The in situ formed AuNPs-MXenes-Apt hybrid not only presented highly efficient recognition of exosomes specifically, but also provide naked catalytic surface with high electrocatalytic activity of gold nanoparticles with predominated (111) facets that significantly improved the ECL signal of luminol. In this way, a highly sensitive ECL biosensor for exosomes detection was constructed ascribing to the synergistic effects of large surface area, excellent conductivity, and catalytic effects of the AuNPs-MXenes-Apt. The detection limit is 30 particles μL–1 for exosomes derived from HeLa cell line, which was over 1000 times lower than that of conventional ELISA method and the linear range was from 102 particles μL–1 to 105 particles μL–1. This ECL sensing platform possessed high selectivity toward exosomes and their surface proteins derived different kinds of tumor cell lines (HeLa cells, OVCAR cells and HepG2 cells), and enabled sensitive and accurate detection of exosomes from human serum, which implied that the ECL biosensor provided a feasible, sensitive, and reliable tool for exosomes detection in exosomes-related clinical diagnostic.
中文翻译：
金纳米粒子的原位形成修饰的Ti3C2 MXenes纳米探针，用于外泌体及其表面蛋白的高灵敏电化学发光检测。
在这项工作中，通过原位形成修饰有适体修饰的Ti 3 C 2 MXenes杂合的金纳米颗粒（AuNPs），开发了一种用于外泌体及其表面蛋白的超灵敏电化学发光（ECL）生物传感器。在这种策略中，外泌体被有效地捕获在外泌体识别的CD63适体修饰的电极界面上。同时，在单层Ti 3 C 2上原位形成金纳米颗粒获得了具有适体的MXene（MXenes-Apt）改性的，其中MXenes既充当还原剂又是稳定剂，并且不涉及额外的还原剂和稳定剂。原位形成的AuNPs-MXenes-Apt杂种不仅表现出对外泌体的高效识别，而且还为裸露的催化表面提供了具有主要（111）晶面的金纳米颗粒的高电催化活性，从而显着改善了鲁米诺的ECL信号。以此方式，构建了用于外泌体检测的高度灵敏的ECL生物传感器，归因于AuNPs-MXenes-Apt的大表面积，优异的电导率和催化作用的协同效应。检出限为30个颗粒μL –1对于来自HeLa细胞系的外泌体，其含量比常规ELISA方法低1000倍以上，线性范围为10 2颗粒μL –1至10 5颗粒μL –1。该ECL传感平台对外泌体具有很高的选择性，其表面蛋白衍生自不同种类的肿瘤细胞系（HeLa细胞，OVCAR细胞和HepG2细胞），并能够灵敏，准确地检测人血清中的外泌体，这意味着ECL生物传感器提供了一种与外泌体相关的临床诊断中外泌体检测的可行，灵敏和可靠的工具。
更新日期：2020-03-24
中文翻译：
金纳米粒子的原位形成修饰的Ti3C2 MXenes纳米探针，用于外泌体及其表面蛋白的高灵敏电化学发光检测。
在这项工作中，通过原位形成修饰有适体修饰的Ti 3 C 2 MXenes杂合的金纳米颗粒（AuNPs），开发了一种用于外泌体及其表面蛋白的超灵敏电化学发光（ECL）生物传感器。在这种策略中，外泌体被有效地捕获在外泌体识别的CD63适体修饰的电极界面上。同时，在单层Ti 3 C 2上原位形成金纳米颗粒获得了具有适体的MXene（MXenes-Apt）改性的，其中MXenes既充当还原剂又是稳定剂，并且不涉及额外的还原剂和稳定剂。原位形成的AuNPs-MXenes-Apt杂种不仅表现出对外泌体的高效识别，而且还为裸露的催化表面提供了具有主要（111）晶面的金纳米颗粒的高电催化活性，从而显着改善了鲁米诺的ECL信号。以此方式，构建了用于外泌体检测的高度灵敏的ECL生物传感器，归因于AuNPs-MXenes-Apt的大表面积，优异的电导率和催化作用的协同效应。检出限为30个颗粒μL –1对于来自HeLa细胞系的外泌体，其含量比常规ELISA方法低1000倍以上，线性范围为10 2颗粒μL –1至10 5颗粒μL –1。该ECL传感平台对外泌体具有很高的选择性，其表面蛋白衍生自不同种类的肿瘤细胞系（HeLa细胞，OVCAR细胞和HepG2细胞），并能够灵敏，准确地检测人血清中的外泌体，这意味着ECL生物传感器提供了一种与外泌体相关的临床诊断中外泌体检测的可行，灵敏和可靠的工具。
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