Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Biosynthesis of Silver Nanoparticles Using Cucumis prophetarum Aqueous Leaf Extract and Their Antibacterial and Antiproliferative Activity Against Cancer Cell Lines
ACS Omega ( IF 3.7 ) Pub Date : 2020-03-02 , DOI: 10.1021/acsomega.0c00155 Hemlata 1 , Prem Raj Meena 2 , Arvind Pratap Singh 2 , Kiran Kumar Tejavath 1
ACS Omega ( IF 3.7 ) Pub Date : 2020-03-02 , DOI: 10.1021/acsomega.0c00155 Hemlata 1 , Prem Raj Meena 2 , Arvind Pratap Singh 2 , Kiran Kumar Tejavath 1
Affiliation
|
Biosynthesized nanoparticles are gaining attention because of biologically active plant secondary metabolites that help in green synthesis and also due to their unique biological applications. This study reports a facile, ecofriendly, reliable, and cost-effective synthesis of silver nanoparticles using the aqueous leaf extract of Cucumis prophetarum (C. prophetarum) and their antibacterial and antiproliferative activity. Silver nanoparticles were biosynthesized using the aqueous leaf extract of C. prophetarum, which acted as a reducing and capping agent. The biosynthesized C. prophetarum silver nanoparticles (Cp-AgNPs) were characterized using different techniques, such as UV–visible spectroscopy, dynamic light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray analysis (EDAX). Phytochemical analysis was performed to determine the phytochemicals responsible for the reduction and capping of the biosynthesized Cp-AgNPs. The antioxidant activity of the biosynthesized nanoparticles was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays. Their antibacterial activity was checked against Staphylococcus aureus (Gram-positive) and Salmonella typhi (Gram-negative) bacteria. The biosynthesized nanoparticles showed dosage-dependent inhibition activity with a significant zone of inhibition and were more effective toward S. typhi as compared to S. aureus. Their antiproliferative activity was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on selected cancer cell lines. The IC50 values of Cp-AgNPs on A549, MDA-MB-231, HepG2, and MCF-7 were found to be 105.8, 81.1, 94.2, and 65.6 μg/mL, respectively, and this showed that the Cp-AgNPs were more potent toward MCF-7 as compared to other cell lines used in this study. This work revealed that the biosynthesized silver nanoparticles using C. prophetarum leaf extract were associated with good antibacterial activity and antiproliferative potential against selected cancer cell lines. The biosynthesized C. prophetarum AgNPs can be further exploited as a potential candidate for antioxidant, antibacterial, and anticancer agents.
中文翻译：
用前积黄瓜水叶提取物生物合成银纳米粒子及其对癌细胞系的抗菌和抗增殖活性
由于具有生物活性的植物次生代谢产物有助于绿色合成，而且还具有独特的生物学应用，生物合成的纳米颗粒正受到关注。这项研究报告了使用黄瓜积水（C. prophetarum）的含水叶提取物轻松，环保，可靠且具有成本效益的方式合成银纳米颗粒及其抗菌和抗增殖活性。使用纳米丙三醇的叶子提取物生物合成银纳米颗粒，该提取物充当还原剂和封端剂。生物合成的丙酸杆菌使用不同的技术对银纳米颗粒（Cp-AgNPs）进行了表征，例如紫外可见光谱，动态光散射（DLS），傅里叶变换红外（FTIR）光谱，X射线衍射（XRD），扫描电子显微镜（SEM）和能量色散X射线分析（EDAX）。进行了植物化学分析，以确定负责生物合成的Cp-AgNPs还原和封端的植物化学物质。生物合成的纳米颗粒的抗氧化活性使用2，2-二苯基-1-吡啶并肼基（DPPH）和3-乙基苯并噻唑啉-6-磺酸（ABTS）测定。检查其对金黄色葡萄球菌（革兰氏阳性）和伤寒沙门氏菌的抗菌活性（革兰氏阴性）细菌。与金黄色葡萄球菌相比，生物合成的纳米颗粒表现出剂量依赖性的抑制活性，具有明显的抑制区域，并且对伤寒沙门氏菌更有效。在选定的癌细胞系上使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）分析评估了它们的抗增殖活性。发现A549，MDA-MB-231，HepG2和MCF-7上Cp-AgNP的IC 50值分别为105.8、81.1、94.2和65.6μg/ mL，这表明Cp-AgNPs为与本研究中使用的其他细胞系相比，对MCF-7的作用更强。这项工作表明使用丙酸丙二醇酯生物合成了银纳米颗粒叶提取物对选定的癌细胞系具有良好的抗菌活性和抗增殖潜力。生物合成的丙酸杆菌AgNPs可以进一步用作抗氧化剂，抗菌剂和抗癌剂的潜在候选物。
更新日期：2020-03-19
中文翻译：
用前积黄瓜水叶提取物生物合成银纳米粒子及其对癌细胞系的抗菌和抗增殖活性
由于具有生物活性的植物次生代谢产物有助于绿色合成，而且还具有独特的生物学应用，生物合成的纳米颗粒正受到关注。这项研究报告了使用黄瓜积水（C. prophetarum）的含水叶提取物轻松，环保，可靠且具有成本效益的方式合成银纳米颗粒及其抗菌和抗增殖活性。使用纳米丙三醇的叶子提取物生物合成银纳米颗粒，该提取物充当还原剂和封端剂。生物合成的丙酸杆菌使用不同的技术对银纳米颗粒（Cp-AgNPs）进行了表征，例如紫外可见光谱，动态光散射（DLS），傅里叶变换红外（FTIR）光谱，X射线衍射（XRD），扫描电子显微镜（SEM）和能量色散X射线分析（EDAX）。进行了植物化学分析，以确定负责生物合成的Cp-AgNPs还原和封端的植物化学物质。生物合成的纳米颗粒的抗氧化活性使用2，2-二苯基-1-吡啶并肼基（DPPH）和3-乙基苯并噻唑啉-6-磺酸（ABTS）测定。检查其对金黄色葡萄球菌（革兰氏阳性）和伤寒沙门氏菌的抗菌活性（革兰氏阴性）细菌。与金黄色葡萄球菌相比，生物合成的纳米颗粒表现出剂量依赖性的抑制活性，具有明显的抑制区域，并且对伤寒沙门氏菌更有效。在选定的癌细胞系上使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）分析评估了它们的抗增殖活性。发现A549，MDA-MB-231，HepG2和MCF-7上Cp-AgNP的IC 50值分别为105.8、81.1、94.2和65.6μg/ mL，这表明Cp-AgNPs为与本研究中使用的其他细胞系相比，对MCF-7的作用更强。这项工作表明使用丙酸丙二醇酯生物合成了银纳米颗粒叶提取物对选定的癌细胞系具有良好的抗菌活性和抗增殖潜力。生物合成的丙酸杆菌AgNPs可以进一步用作抗氧化剂，抗菌剂和抗癌剂的潜在候选物。
京公网安备 11010802027423号