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Osmotic-Tension-Induced Membrane Lateral Organization.
Langmuir ( IF 3.7 ) Pub Date : 2020-03-16 , DOI: 10.1021/acs.langmuir.9b03893 Nichaporn Wongsirojkul 1, 2 , Naofumi Shimokawa 1 , Pakorn Opaprakasit 2 , Masahiro Takagi 1 , Tsutomu Hamada 1
Langmuir ( IF 3.7 ) Pub Date : 2020-03-16 , DOI: 10.1021/acs.langmuir.9b03893 Nichaporn Wongsirojkul 1, 2 , Naofumi Shimokawa 1 , Pakorn Opaprakasit 2 , Masahiro Takagi 1 , Tsutomu Hamada 1
Affiliation
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Alteration of lipid raft organization manifesting as phase separation is important for cellular processes, such as signaling and trafficking. Such behaviors and dynamics of lipid membranes can be affected by external stimuli including both physical and chemical stimuli. In this study, we focused on osmotic-tension-induced phase separation. The effects of osmotic tension on the phase behaviors of vesicles consisting of dioleoylphosphocholine (DOPC)/dipalmitoylphosphocholine (DPPC)/cholesterol (Chol) were quantitatively studied at different temperatures by fluorescence microscopy. We determined the ternary phase diagrams and found that tension leads to a shift in the miscibility temperature. Cholesterol plays a key role in determining the extent of this shift. In addition, we found that osmotic tension can enhance the line tension. The physicochemical mechanism of osmotic-pressure-induced phase separation is discussed.
中文翻译：
渗透张力诱导的膜横向组织。
表现为相分离的脂质筏组织的改变对于细胞过程，例如信号传导和运输非常重要。脂质膜的这种行为和动力学会受到包括物理和化学刺激在内的外部刺激的影响。在这项研究中，我们专注于渗透压诱导的相分离。通过荧光显微镜定量研究了渗透压对由二油酰基磷酸胆碱（DOPC）/二棕榈酰基磷酸胆碱（DPPC）/胆固醇（Chol）组成的囊泡的相行为的影响。我们确定了三元相图，发现张力导致混溶温度的变化。胆固醇在确定这种变化的程度中起关键作用。另外，我们发现渗透压可以增强线张力。
更新日期：2020-03-16
中文翻译：
渗透张力诱导的膜横向组织。
表现为相分离的脂质筏组织的改变对于细胞过程，例如信号传导和运输非常重要。脂质膜的这种行为和动力学会受到包括物理和化学刺激在内的外部刺激的影响。在这项研究中，我们专注于渗透压诱导的相分离。通过荧光显微镜定量研究了渗透压对由二油酰基磷酸胆碱（DOPC）/二棕榈酰基磷酸胆碱（DPPC）/胆固醇（Chol）组成的囊泡的相行为的影响。我们确定了三元相图，发现张力导致混溶温度的变化。胆固醇在确定这种变化的程度中起关键作用。另外，我们发现渗透压可以增强线张力。
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