Bioelectricity generation, by Shewanella oneidensis (S. oneidensis) MR-1, has become particularly alluring, thanks to its extraordinary prospects for energy production, pollution treatment, and biosynthesis. Attempts to improve its technological output by modification of S. oneidensis MR-1 remains complicated, expensive and inefficient. Herein, we report on the augmentation of S. oneidensis MR-1 with carbon dots (CDs). The CDs-fed cells show accelerated extracellular electron transfer and metabolic rate, with increased intracellular charge, higher adenosine triphosphate level, quicker substrate consumption and more abundant extracellular secretion. Meanwhile, the CDs promote cellular adhesion, electronegativity, and biofilm formation. In bioelectrical systems the CDs-fed cells increase the maximum current value, 7.34 fold, and power output, 6.46 fold. The enhancement efficacy is found to be strongly dependent on the surface charge of the CDs. This work demonstrates a simple, cost-effective and efficient route to improve bioelectricity generation of S. oneidensis MR-1, holding promise in all relevant technologies.

由于其在能源生产，污染处理和生物合成方面的非凡前景，由Shewanella oneidensis（S. oneidensis）MR-1进行的生物发电已变得特别诱人。试图通过对链球菌S.oneidensis MR-1的改良来提高其技术产量仍然复杂，昂贵且效率低下。在此，我们报道了沙门氏菌的扩增带有碳点（CD）的MR-1。由CDs喂养的细胞显示出加速的细胞外电子转移和代谢速率，细胞内电荷增加，三磷酸腺苷水平更高，底物消耗更快，细胞外分泌更丰富。同时，CD促进细胞粘附，电负性和生物膜形成。在生物电系统中，由CD供电的电池将最大电流值提高了7.34倍，将功率输出提高了6.46倍。发现增强功效强烈依赖于CD的表面电荷。这项工作展示了一种简单，具有成本效益的有效途径来改善沙棘葡萄球菌MR-1的生物电发电，在所有相关技术中均具有前景。