LWT - Food Science and Technology ( IF 6.0 ) Pub Date : 2020-03-14 , DOI: 10.1016/j.lwt.2020.109280 Mei-Jun Ye , Qing-Ling Xu , Hong-Yan Tang , Wen-Yi Jiang , Dong-Xiao Su , Shan He , Qing-Zhu Zeng , Yang Yuan
A novel selenium nanoparticles (SeNPs) was prepared by using peanut meal peptides mixture (Ppm) as a stabilizer in ascorbic acid reduction sodium selenite system. It was found that Ppm prepared by alcalase and neutral protease had high degree of hydrolysis and antioxidant activity and the obtained Ppm-SeNPs solution were stabilized at 4 °C for 60 days with an average particle size of 140 nm. The interaction and morphological characterization revealed that Ppm-SeNPs were spherical and wrapped by Ppm successfully which may contributed to electrostatic and hydrophobic interaction. The results of infrared spectroscopy indicated that the N- and O- functional groups on the Ppm were combined with SeNPs. Moreover, ascorbic acid and cysteine were compared as the reducing agents for reducing sodium selenite, it turned out that the stability of Ppm-SeNPs obtained by ascorbic acid as reducing agent was better than that of cysteine.
中文翻译:
新型花生粉与硒胶体复合物的研制与稳定性
在抗坏血酸还原亚硒酸钠体系中,以花生粕多肽混合物(Ppm)为稳定剂,制备了一种新型硒纳米粒子(SeNPs)。发现由碱性蛋白酶和中性蛋白酶制备的Ppm具有高度的水解和抗氧化活性,并且将获得的Ppm-SeNPs溶液在4℃下稳定60天,平均粒径为140nm。相互作用和形态学表征表明,Ppm-SeNPs呈球形并被Ppm成功包裹,这可能有助于静电和疏水相互作用。红外光谱的结果表明,Ppm上的N-和O-官能团与SeNPs结合在一起。此外,比较了抗坏血酸和半胱氨酸作为还原剂,可还原亚硒酸钠,