Construction of a Target-Initiated, Enzyme-Free DNA Cascade Circuit for Amplified Detection of Mercury,ACS Applied Bio Materials

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Construction of a Target-Initiated, Enzyme-Free DNA Cascade Circuit for Amplified Detection of Mercury
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-03-12 , DOI: 10.1021/acsabm.0c00142
Chao Xing ₁ , Ziyi Chen ₂ , Cheng Zhang ₁ , Jun Wang ₁ , Chunhua Lu ₂

Affiliation

Here, we designed a target-initiated, enzyme-free DNA cascade circuit for highly selective and sensitive detection of Hg²⁺ with dual amplification based on concatenated fuel stimulant powered (FSP) and catalytic hairpin assembly (CHA) circuits. In a typical FSP–CHA procedure, in the presence of Hg²⁺, the invading DNA activated the upstream FSP circuit to release the hidden strands containing multiple trigger units, which subsequently motivated the downstream CHA circuit to drive the cross-opening of two hairpin substrates, generating tremendously amplified fluorescence signals. A good linearity between the fluorescence intensity and Hg²⁺ concentrations was observed in the range from 0.2 nM to 20 nM. The proposed system showed great applicability for sensing Hg²⁺ in environmentally relevant water samples.

中文翻译：

构建目标启动、无酶 DNA 级联电路，用于放大汞检测

在这里，我们设计了一种靶向启动的无酶 DNA 级联电路，用于高选择性和灵敏地检测 Hg ²⁺，并基于串联的燃料刺激剂供电 (FSP) 和催化发夹组装 (CHA) 电路进行双重放大。在典型的 FSP-CHA 程序中，在 Hg ²⁺存在的情况下，入侵的 DNA 激活上游 FSP 电路释放包含多个触发单元的隐藏链，随后驱动下游 CHA 电路驱动两个发夹交叉打开底物，产生极大放大的荧光信号。荧光强度和 Hg ^{2+之间具有良好的线性}在 0.2 nM 至 20 nM 的范围内观察到浓度。所提出的系统在检测环境相关水样中的 Hg ^{2+方面表现出极大的适用性。}

更新日期：2020-04-23

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