细胞表面能影响微藻生物膜的结构。,Langmuir

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细胞表面能影响微藻生物膜的结构。
Langmuir ( IF 3.7 ) Pub Date : 2020-03-20 , DOI: 10.1021/acs.langmuir.0c00274
Xinru Zhang _{1,

2} , Hao Yuan ₁ , Yi Wang ₁ , Libo Guan ₁ , Ziyi Zeng ₁ , Zeyi Jiang _{1,

3} , Xinxin Zhang _{1,

3}

Affiliation

基于微藻生物膜的培养系统在环境工程和生物技术中具有广泛的应用。生物膜结构对于微藻生物膜中养分，气体和信号分子的运输至关重要。这项工作旨在了解细胞表面能（SE）对微藻生物膜结构的影响。三种微藻被用作细胞模型的研究：小球藻，SP，微绿球藻和小球藻。首先，通过介导生物膜培养条件，我们获得了小球藻。SE分别为40.4±1.5、44.7±1.0和62. 7±1.2 mJ / m ^2的细胞，N。oculata细胞，SE分别为47.7±0.5、41.1±1.0和62.6±1.2 mJ / m ^2的细胞，和C.pyrenoidosa细胞，SE分别为64.0±0.6、62.1±0.7和62.8±0.6 mJ / m ²。然后，基于生物膜结构的表征，我们发现细胞SE可以显着影响微藻生物膜结构。当细胞SEs为40至50mJ / m ^2时，微藻细胞形成具有大量开放空隙的异质生物膜，并且生物膜孔隙率高于20％。或者，当单元SE的范围从50到65 mJ / m ^2时，细胞形成了平坦，均匀的生物膜，孔隙率低于20％。最后，基于热力学理论，通过分析细胞之间的共黏附能，解释了细胞SE对生物膜结构的影响机理。这项研究对于理解影响生物膜结构的因素具有重要意义。

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Cell Surface Energy Affects the Structure of Microalgal Biofilm.

Microalgae biofilm-based culture systems have wide applications in environmental engineering and biotechnology. Biofilm structure is critical for the transport of nutrients, gas, and signaling molecules in a microalgal biofilm. This work aims to understand the influence of cell surface energy (SE) on the microalgal biofilm structure. Three microalgae species were used as model cells in the study: Chlorella sp., Nannochloris oculata, and Chlorella pyrenoidosa. First, by mediating biofilm culture conditions, we obtained Chlorella sp. cells with SEs of 40.4 ± 1.5, 44.7 ± 1.0, and 62. 7 ± 1.2 mJ/m², N. oculata cells with SEs of 47.7 ± 0.5, 41.1 ± 1.0, and 62.6 ± 1.2 mJ/m², and C. pyrenoidosa cells with SEs of 64.0 ± 0.6, 62.1 ± 0.7, and 62.8 ± 0.6 mJ/m². Then, based on the characterizations of biofilm structures, we found that cell SE can significantly affect the microalgae biofilm structure. When the cell SEs ranged from 40 to 50 mJ/m², the microalgae cells formed heterogeneous biofilms with a large number of open voids, and the biofilm porosity was higher than 20%. Alternatively, when the cell SEs ranged from 50 to 65 mJ/m², the cells formed a flat, homogeneous biofilm with the porosity lower than 20%. Finally, the influencing mechanism of cell SE on biofilm structure was interpreted based on the thermodynamic theory via analyzing the co-adhesion energy between cells. The study has important implications in understanding factors that influence the biofilm structures.

更新日期：2020-03-21

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