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Transferrin-Conjugated Red Blood Cell Membrane-Coated Poly(lactic-co-glycolic acid) Nanoparticles for the Delivery of Doxorubicin and Methylene Blue
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2020-03-10 , DOI: 10.1021/acsanm.0c00502 Anil Parsram Bidkar 1 , Pallab Sanpui 2 , Siddhartha Sankar Ghosh 1, 3
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2020-03-10 , DOI: 10.1021/acsanm.0c00502 Anil Parsram Bidkar 1 , Pallab Sanpui 2 , Siddhartha Sankar Ghosh 1, 3
Affiliation
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Herein, we demonstrate the preparation of transferrin (TF) receptor-targeted RBC membrane coated nanoparticles (NPs) to achieve chemotherapeutic and photodynamic effects. The TF conjugated RBC membranes were developed to incorporate doxorubicin (Dox) and methylene blue (MB) loaded PLGA NPs by the extrusion method to produce TF-DoxMB NPs. Characterizations revealed that the NPs were spherical in size (106 ± 23 nm) with a zeta potential (ζ) of −12.6 mV. Coating of the TF conjugated membrane on the NP surface resulted in targeted delivery of the drugs to the TF receptor expressing cancer cells. Following this, exposure of the treated cells with red laser generated reactive oxygen species from photosensitizer MB. Targeted delivery of Dox and MB with subsequent laser irradiation selectively annihilated cancer cells. Viability assays and combination index (CI) analysis revealed synergistic action of the chemo- and photodynamic therapy. Interestingly, the antiproliferative effect of Dox and ROS produced due to photosensitization led to DNA damage mediated apoptosis in HeLa and MCF-7 cells. Targeting efficiency of the TF-DoxMB NPs was quite intriguing in tumor spheroids due to the synergistic action of Dox and MB.
中文翻译：
转铁蛋白-共轭红细胞膜涂覆的聚（乳酸-共-glycolic酸）纳米颗粒为多柔比星和亚甲基蓝的交付
在这里，我们演示了转铁蛋白（TF）受体靶向的RBC膜包被的纳米颗粒（NPs）的制备，以实现化学治疗和光动力作用。通过挤出方法开发了TF共轭RBC膜，以掺入阿霉素（Dox）和亚甲蓝（MB）的PLGA NP，从而生产TF-DoxMB NP。表征表明，NP呈球形（106±23 nm），ζ电位（ζ）为-12.6 mV。TF共轭膜在NP表面的覆盖导致药物靶向递送至表达TF受体的癌细胞。此后，用红色激光照射处理过的细胞会从光敏剂MB产生活性氧。Dox和MB的靶向递送以及随后的激光照射选择性消灭了癌细胞。可行性分析和组合指数（CI）分析显示了化学和光动力疗法的协同作用。有趣的是，由于光敏作用而产生的Dox和ROS的抗增殖作用导致DNA损伤介导的HeLa和MCF-7细胞凋亡。由于Dox和MB的协同作用，TF-DoxMB NP的靶向效率在肿瘤球体中非常吸引人。
更新日期：2020-03-10
中文翻译：
转铁蛋白-共轭红细胞膜涂覆的聚（乳酸-共-glycolic酸）纳米颗粒为多柔比星和亚甲基蓝的交付
在这里，我们演示了转铁蛋白（TF）受体靶向的RBC膜包被的纳米颗粒（NPs）的制备，以实现化学治疗和光动力作用。通过挤出方法开发了TF共轭RBC膜，以掺入阿霉素（Dox）和亚甲蓝（MB）的PLGA NP，从而生产TF-DoxMB NP。表征表明，NP呈球形（106±23 nm），ζ电位（ζ）为-12.6 mV。TF共轭膜在NP表面的覆盖导致药物靶向递送至表达TF受体的癌细胞。此后，用红色激光照射处理过的细胞会从光敏剂MB产生活性氧。Dox和MB的靶向递送以及随后的激光照射选择性消灭了癌细胞。可行性分析和组合指数（CI）分析显示了化学和光动力疗法的协同作用。有趣的是，由于光敏作用而产生的Dox和ROS的抗增殖作用导致DNA损伤介导的HeLa和MCF-7细胞凋亡。由于Dox和MB的协同作用，TF-DoxMB NP的靶向效率在肿瘤球体中非常吸引人。
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