Dietary flavonoid myricetin inhibits invasion and migration of radioresistant lung cancer cells (A549-IR) by suppressing MMP-2 and MMP-9 expressions through inhibition of the FAK-ERK signaling pathway.,Food Science & Nutrition

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Dietary flavonoid myricetin inhibits invasion and migration of radioresistant lung cancer cells (A549-IR) by suppressing MMP-2 and MMP-9 expressions through inhibition of the FAK-ERK signaling pathway.
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2020-03-09 , DOI: 10.1002/fsn3.1495
Hye R Kang ₁ , Jeong Y Moon ₂ , Meran K Ediriweera ₂ , Yeon W Song ₃ , Moonjae Cho _{1,

4} , Dharanibalan Kasiviswanathan ₄ , Somi K Cho _{1,

2,

3,

5}

Affiliation

Myricetin is a commonly found dietary flavonoid. In the present study, we investigated the effects of myricetin on migration and invasion of radioresistant lung cancer cells (A549‐IR). Transcriptome analysis of A549‐IR cells identified several differentially expressed genes (DEGs) in A549‐IR cells compared to parental A549 cells. Functional enrichment analysis revealed that most of the DEGs were linked with PI3K‐AKT signaling, proteoglycans, focal adhesion, and ECM–receptor interactions. A549‐IR cells demonstrated enhanced migratory potential with increased expression of vimentin, snail and slug, and reduced expression of E‐cadherin. A549‐IR cells exposed to myricetin displayed reduced migration and suppressed MMP‐2 and MMP‐9 expression. Notably, myricetin inhibited the phosphorylation of focal adhesion kinase (FAK) and altered the F‐actin/G‐actin ratio in A549‐IR cells, without modulation of EMT markers. These findings suggest that myricetin can inhibit migration of A549‐IR cells by suppressing MMP‐2 and MMP‐9 expressions through inhibition of the FAK‐ERK signaling pathway.

中文翻译：

膳食类黄酮杨梅素通过抑制 FAK-ERK 信号通路来抑制 MMP-2 和 MMP-9 的表达，从而抑制放射抗性肺癌细胞 (A549-IR) 的侵袭和迁移。

杨梅素是一种常见的膳食类黄酮。在本研究中，我们研究了杨梅素对放射抗性肺癌细胞（A549-IR）迁移和侵袭的影响。 A549-IR 细胞的转录组分析发现，与亲代 A549 细胞相比，A549-IR 细胞中存在几个差异表达基因 (DEG)。功能富集分析显示，大多数 DEG 与 PI3K-AKT 信号传导、蛋白聚糖、粘着斑和 ECM 受体相互作用有关。 A549-IR 细胞表现出增强的迁移潜力，波形蛋白、蜗牛和蛞蝓的表达增加，而 E-钙粘蛋白的表达减少。暴露于杨梅素的 A549-IR 细胞显示迁移减少并抑制 MMP-2 和 MMP-9 表达。值得注意的是，杨梅素抑制粘着斑激酶 (FAK) 的磷酸化并改变 A549-IR 细胞中的 F-肌动蛋白/G-肌动蛋白比率，而不调节 EMT 标记。这些发现表明，杨梅素可以通过抑制 FAK-ERK 信号通路来抑制 MMP-2 和 MMP-9 的表达，从而抑制 A549-IR 细胞的迁移。

更新日期：2020-03-09

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