Exploration of the Substrate Diversity of Leucoanthocyanidin Reductases,Journal of Agricultural and Food Chemistry

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Exploration of the Substrate Diversity of Leucoanthocyanidin Reductases
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-03-20 , DOI: 10.1021/acs.jafc.9b06353
Lingjie Zhang ₁ , Peiqiang Wang _{2,

3} , Xue Ma ₁ , Wenyan Zhao ₁ , Ming Li ₁ , Shengbo Yao _{2,

4} , Yajun Liu ₁ , Liping Gao ₁ , Tao Xia _{2,

4}

Affiliation

Proanthocyanidins (PAs) are mainly composed of epicatechin (EC) or catechin (C) subunits. C-type catechins (C and GC) are generally considered to be catalyzed by leucocyanidin reductase (LAR). In this study, we re-evaluated the function of LAR. LcLAR1 was isolated from Lotus corniculatus, which is rich in C-type catechins. Overexpression of LcLAR1 in tobacco resulted in a significantly increased content of EC and EC-glucoside. Overexpression of LcLAR1 in Arabidopsis thaliana promoted the accumulation of soluble PAs, including EC, PA dimers, and PA trimers. However, in the transgenic ans mutant overexpressing LcLAR1, the contents of C and C-glucoside were increased. In addition, overexpression of LcLAR1 in L. corniculatus resulted in a significant increase of C levels. Taken together, the products of LcLAR1 depended on the substrates, which revealed the substrate diversity of LcLAR1. Our study provides new insights into the flavonoid pathway, especially the role of LAR.

中文翻译：

白花色素还原酶底物多样性的探索

原花青素（PAs）主要由表儿茶素（EC）或儿茶素（C）亚基组成。通常认为C型儿茶素（C和GC）被亮蓝花色素还原酶（LAR）催化。在这项研究中，我们重新评估了LAR的功能。LcLAR1是从富含C型儿茶素的Lotus corniculatus中分离出来的。烟草中LcLAR1的过表达导致EC和EC-葡萄糖苷的含量显着增加。LcLAR1在拟南芥中的过表达促进了可溶性PA的积累，包括EC，PA二聚体和PA三聚体。然而，在转基因ans突变体中过表达LcLAR1，C和C-葡萄糖苷的含量增加。此外，过度LcLAR1在L.百脉导致C水平的显著上升。两者合计，Lc LAR1的产物取决于底物，这揭示了Lc LAR1的底物多样性。我们的研究为类黄酮途径，特别是LAR的作用提供了新见解。

更新日期：2020-03-21

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