X射线光电子能谱（XPS）对纳米复合物的表面分析,ACS Biomaterials Science & Engineering

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X射线光电子能谱（XPS）对纳米复合物的表面分析
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2017-05-07 00:00:00 , DOI: 10.1021/acsbiomaterials.7b00040
Efrat Korin ₁ , Natalya Froumin ₁ , Smadar Cohen ₁

Affiliation

由单个分子通过非共价相互作用自发连接的自组装纳米复合物，由于其独特的生物启发性质（反应性，动力学等），最近已成为常规控制药物递送系统的通用替代品。此类纳米复合物的表征通常包括其尺寸分布，表面电荷，形态，药物截留效率以及使用共定位研究验证纳米复合物中标记组分的共存。较少见的是直接检查共组装纳米复合物中不同组分之间的分子相互作用，尤其是在由吸湿性组分组成的纳米复合物中，因为仍然缺乏便利的方法。这里，我们将在UHV下干燥过夜的吸湿性样品后，通过X射线光电子能谱（XPS）确定表面成分和化学键的详细实验方案。我们用这种方法研究了二元钙的表面化学沉积在晶圆上的透明质酸硫酸盐（HAS）-Ca ²⁺ -siRNA的²⁺ -siRNA纳米复合物和三元纳米复合物。值得注意的是，我们证明了该协议可用于研究传统XPS仪器的表面组成和沉积的纳米复合物的相互作用，并且只需要相对少量的纳米复合物悬浮液即可。

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Surface Analysis of Nanocomplexes by X-ray Photoelectron Spectroscopy (XPS)

Self-assembled nanocomplexes composed of individual molecules that spontaneously connect via noncovalent interactions have recently emerged as versatile alternatives to conventional controlled drug delivery systems because of their unique bioinspired properties (responsiveness, dynamics, etc.). Characterization of such nanocomplexes typically includes their size distribution, surface charge, morphology, drug entrapment efficiency, and verification of the coexistence of labeled components within the nanocomplexes using a colocalization study. Less common is the direct examination of the molecular interactions between the different components in the coassembled nanocomplex, especially in nanocomplexes composed of hygroscopic components, because convenient methods are still lacking. Here, we present a detailed experimental protocol for determining the surface composition and the chemical bonds by X-ray photoelectron spectroscopy (XPS) after drying the deposit hygroscopic sample overnight under UHV. We applied this method to investigate the surface chemistry of binary Ca²⁺-siRNA nanocomplexes and ternary nanocomplexes of hyaluronan-sulfate (HAS)-Ca²⁺-siRNA, deposited on a wafer. Notably, we showed that the protocol can be implemented to study the surface composition and interactions of the deposited nanocomplexes with a traditional XPS instrument, and it requires only a relatively small amount of the nanocomplex suspension.

更新日期：2017-05-07

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