拟南芥叶片发育中由不对称叶1（AS1）和AS2调控的遗传网络：KNOX基因控制五个形态事件,The Plant Journal

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拟南芥叶片发育中由不对称叶1（AS1）和AS2调控的遗传网络：KNOX基因控制五个形态事件
The Plant Journal ( IF 6.2 ) Pub Date : 2009-11-04 , DOI: 10.1111/j.1365-313x.2009.04033.x
Masaya Ikezaki , Mikiko Kojima , Hitoshi Sakakibara , Shoko Kojima , Yoshihisa Ueno , Chiyoko Machida , Yasunori Machida

拟南芥的不对称叶1（as1）和as2突变体表现出多效性表型。这些突变体增强了许多基因的表达，包括三个1类KNOTTED样同源异型盒（KNOX）基因（BP，KNAT2和KNAT6）和ETTIN / ARF3。在本研究中，我们试图确定的表型特征AS1和AS2通过异位表达生成的突变体KNOX基因，使用多种丧失功能的突变KNOX基因以及as1和as2。我们的研究结果表明，第1类KNOX基因的异位表达导致叶片大小减少，萼片和花瓣大小减少，中脉的形成减少，不定根形成的抑制和晚开花。我们的研究结果还表明，叶大小的减少和开花晚期是由KNOX基因抑制了as1和as2中的赤霉素（GA）途径引起的植物。然而，不太明显的中脉的形成和不定根的形成的抑制与GA途径无关。KNOX基因的多个突变不能挽救as1和as2植物叶片上叶裂的不对称形成，高阶静脉的较低复杂性以及不定芽的形成频率。因此，这些特征必须受到其他基因的控制，其中在as1和as2突变体中表达增强。

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Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

The asymmetric leaves 1 (as1) and as2 mutants of Arabidopsis thaliana exhibit pleiotropic phenotypes. Expression of a number of genes, including three class‐1 KNOTTED‐like homeobox (KNOX) genes (BP, KNAT2 and KNAT6) and ETTIN/ARF3, is enhanced in these mutants. In the present study, we attempted to identify the phenotypic features of as1 and as2 mutants that were generated by ectopic expression of KNOX genes, using multiple loss‐of‐function mutations of KNOX genes as well as as1 and as2. Our results revealed that the ectopic expression of class‐1 KNOX genes resulted in reductions in the sizes of leaves, reductions in the size of sepals and petals, the formation of a less prominent midvein, the repression of adventitious root formation and late flowering. Our results also revealed that the reduction in leaf size and late flowering were caused by the repression, by KNOX genes, of a gibberellin (GA) pathway in as1 and as2 plants. The formation of a less prominent midvein and the repression of adventitious root formation were not, however, related to the GA pathway. The asymmetric formation of leaf lobes, the lower complexity of higher‐ordered veins, and the elevated frequency of adventitious shoot formation on leaves of as1 and as2 plants were not rescued by multiple mutations in KNOX genes. These features must, therefore, be controlled by other genes in which expression is enhanced in the as1 and as2 mutants.

更新日期：2009-11-04

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