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A novel reaction-based fluorescence probe for rapid imaging of HClO in live cells, animals, and injured liver tissues.
Talanta ( IF 5.6 ) Pub Date : 2020-03-05 , DOI: 10.1016/j.talanta.2020.120901 Yingzhen Deng 1 , Shumin Feng 1 , Qingfeng Xia 1 , Shengyi Gong 1 , Guoqiang Feng 1
Talanta ( IF 5.6 ) Pub Date : 2020-03-05 , DOI: 10.1016/j.talanta.2020.120901 Yingzhen Deng 1 , Shumin Feng 1 , Qingfeng Xia 1 , Shengyi Gong 1 , Guoqiang Feng 1
Affiliation
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Hypochlorous acid (HClO) is an important bioactive molecule, playing vital roles in a large number of physiological and pathological processes. Abnormal concentration of HClO in vivo has close contact with many diseases including inflammatory diseases and cancer. For bioimaging HClO, a new colorimetric and turn-on near-infrared (NIR) fluorescence probe (DDAO-ClO) was designed and synthesized in this work through a specific reaction of HClO with dimethylthiocarbamate. DDAO-ClO proved to show distinct and highly selective colorimetric and NIR fluorescence responses for HClO with fast response time (<3 s) and high sensitivity (LOD = 7.3 nM) in vitro. After confirming the excellent in vitro sensing ability, imaging HClO with DDAO-ClO in living HeLa cells, MCF-7 cells, zebrafish, and mice was all successfully demonstrated. And with this probe, it was further discovered that more endogenous HClO was produced in injured mice liver tissues, which demonstrates that DDAO-ClO not only is effective for in vivo detection of HClO but also has a broad application prospect.
中文翻译：
一种新颖的基于反应的荧光探针，用于活细胞，动物和受伤的肝组织中的HClO快速成像。
次氯酸（HClO）是重要的生物活性分子，在许多生理和病理过程中起着至关重要的作用。体内HClO浓度异常与包括炎症性疾病和癌症在内的许多疾病密切接触。对于生物成像HClO，设计了一种新的比色和开启式近红外（NIR）荧光探针（DDAO-ClO），并通过HClO与二甲基硫代氨基甲酸酯的特定反应合成了该探针。事实证明，DDAO-ClO对HClO表现出独特且高度选择性的比色和NIR荧光响应，体外响应时间短（<3 s），灵敏度高（LOD = 7.3 nM）。在确认了出色的体外传感能力后，用DDAO-ClO对活的HeLa细胞，MCF-7细胞，斑马鱼和小鼠中的HClO进行了成像。有了这个探针，
更新日期：2020-03-05
中文翻译：
一种新颖的基于反应的荧光探针，用于活细胞，动物和受伤的肝组织中的HClO快速成像。
次氯酸（HClO）是重要的生物活性分子，在许多生理和病理过程中起着至关重要的作用。体内HClO浓度异常与包括炎症性疾病和癌症在内的许多疾病密切接触。对于生物成像HClO，设计了一种新的比色和开启式近红外（NIR）荧光探针（DDAO-ClO），并通过HClO与二甲基硫代氨基甲酸酯的特定反应合成了该探针。事实证明，DDAO-ClO对HClO表现出独特且高度选择性的比色和NIR荧光响应，体外响应时间短（<3 s），灵敏度高（LOD = 7.3 nM）。在确认了出色的体外传感能力后，用DDAO-ClO对活的HeLa细胞，MCF-7细胞，斑马鱼和小鼠中的HClO进行了成像。有了这个探针，
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