NanoLuc (NLuc)-furimazine bioluminescence system offers several advantages over established systems, including improved stability, smaller size, and >150-fold enhancement in bioluminescence. Herein, we designed and synthesized a series of bioluminescent substrates with varying at the C-6 position of furimazine for NLuc-furimazine bioluminescence system. Among all derivatives, compounds A6 and A11 provided excellent bioluminescence characteristics compared with furimazine in vitro and in vivo. We believe that these new NLuc substrates can broaden the application of NLuc bioluminescence techniques, especially in vivo bioluminescent imaging.

与已建立的系统相比，NanoLuc（NLuc）-呋喃嗪生物发光系统具有多个优势，包括改进的稳定性，更小的尺寸以及生物发光的> 150倍增强。在这里，我们设计和合成了一系列生物发光底物，用于NLuc-呋喃嗪生物发光系统，其呋喃嗪的C-6位置不同。在所有衍生物中，与呋喃嗪在体外和体内相比，化合物A6和A11具有出色的生物发光特性。我们相信这些新的NLuc底物可以拓宽NLuc生物发光技术的应用，尤其是体内生物发光成像。