Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase.,FEBS Open Bio

当前位置： X-MOL 学术 › FEBS Open Bio › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Serpin7 controls egg diapause of migratory locust (Locusta migratoria) by regulating polyphenol oxidase.
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-03-24 , DOI: 10.1002/2211-5463.12825
Jun Chen ₁ , Dongnan Cui ₁ , Hidayat Ullah _{1,

2} , Kun Hao ₁ , Xiongbing Tu ₁ , Zehua Zhang ₁

Affiliation

Diapause is a state of arrested growth, which allows insects to adapt to diverse environments. Serine protease inhibitors (serpins) play an important role in various physiological processes, including blood coagulation, fibrinolysis, development, complement activation and extracellular matrix remodeling. We hypothesized that serpin may affect energy metabolism and thereby control diapause of migratory locust (Locusta migratoria) embryos by regulating protease cascades. A total of seven nonredundant serpin genes (named serpin1–serpin7) of L. migratoria were obtained through transcriptomic analysis. We further performed label‐free proteomic sequencing and analysis of diapause and nondiapause eggs of L. migratoria, revealing significant differences in serpin7 expression. A significant reduction in diapause rate under the short photoperiod was observed in insects treated with serpin7 double‐stranded RNA. Furthermore, knockdown of the serpin7 gene resulted in significant upregulation of the activity of polyphenol oxidase. We therefore propose that the observed serpin7 gene plays a crucial role in diapause, suggesting that control of energy metabolism may have potential as a future strategy for the reproductive control of insect pests.

中文翻译：

Serpin7通过调节多酚氧化酶来控制卵对蝗虫的滞育。

滞育是阻止生长的状态，它使昆虫能够适应各种环境。丝氨酸蛋白酶抑制剂（serpins）在各种生理过程中起着重要作用，包括血液凝固，纤维蛋白溶解，发育，补体激活和细胞外基质重塑。我们假设丝氨酸蛋白酶抑制剂可能会影响能量代谢，从而通过调节蛋白酶级联反应来控制蝗虫滞育（Locusta migratoria）胚胎。通过转录组分析，总共获得了七个L. migratoria的非冗余丝氨酸蛋白酶抑制蛋白基因（称为serpin1 – serpin7）。我们进一步进行了无标记的蛋白质组测序，并分析了L. migratoria的滞育和非滞育卵。，揭示了serpin7表达的显着差异。在用serpin7双链RNA处理的昆虫中，在短光周期下的滞育率显着降低。此外，serpin7基因的敲低导致多酚氧化酶活性的显着上调。因此，我们建议观察到的serpin7基因在滞育中起关键作用，这表明控制能量代谢可能作为潜在的害虫繁殖控制策略。

更新日期：2020-03-24

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南