与双瓣樱草（蔷薇科）的花瓣状萼片形成相关的PISTILLATA直向同源物的表达模式和功能表征。,Frontiers in Plant Science

当前位置： X-MOL 学术 › Front. Plant Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

与双瓣樱草（蔷薇科）的花瓣状萼片形成相关的PISTILLATA直向同源物的表达模式和功能表征。
Frontiers in Plant Science ( IF 4.1 ) Pub Date : 2020-01-17 , DOI: 10.3389/fpls.2019.01685
Yan Xia _{1,

2} , Min Shi _{1,

2} , Weiwei Chen _{1,

2} , Ruoqian Hu ₂ , Danlong Jing _{1,

2} , Di Wu _{1,

2} , Shuming Wang _{1,

2} , Qingfen Li ₃ , Honghong Deng _{1,

2} , Qigao Guo _{1,

2} , Guolu Liang _{1,

2}

Affiliation

一种新的种质是揭示花器官转化基础的分子机制的一种新种质，它的一个表型是双花的日本堇（Eriobotrya japonica）。在这里，我们分析了EjPI的序列，表达模式和功能特性，EjPI从遗传上同源的单朵和双朵日本粳稻中编码了一种称为PISTILLATA ortholog的B类花卉同源蛋白质。系统发育分析表明，EjPI基因被分配给玫瑰色PI / GLO谱系。蛋白质序列比对分析表明，EjPI具有M，I，K和C的典型域，并在C端区域包含独特的PI基序。与星号PI / GLO谱系相比，EjPI的K1和K3子域都包含一个氨基酸差异。EjPI的亚细胞定位被确定为在细胞核中。表达模式分析表明，EjPI不仅在单花粳稻的花瓣，花丝和花药中表达，而且在双花粳稻的花瓣状萼片中表达。同时，EjPI转录水平与萼片内的花瓣状区域之间存在高度相关性。此外，35S :: EjPI转基因野生型拟南芥引起第一轮萼片向花瓣状萼片的同源转化。EjPI在转基因pi-1突变拟南芥中的异位表达拯救了正常的花瓣和雄蕊。这些结果表明EjPI的表达模式与花瓣状萼片的形成有关。我们的研究提供了EjPI在生物技术工程中创建花瓣状萼片或调节被子植物花器官身份的潜在应用。

"点击查看英文标题和摘要"

Expression Pattern and Functional Characterization of PISTILLATA Ortholog Associated With the Formation of Petaloid Sepals in Double-Flower Eriobotrya japonica (Rosaceae).

Double-flower Eriobotrya japonica, of which one phenotype is homeotic transformation of sepals into petals, is a new germplasm for revealing the molecular mechanisms underlying the floral organ transformation. Herein, we analyzed the sequence, expression pattern and functional characterization of EjPI, which encoded a B-class floral homeotic protein referred to as PISTILLATA ortholog, from genetically cognate single-flower and double-flower E. japonica. Phylogenetic analysis suggested that the EjPI gene was assigned to the rosids PI/GLO lineage. Analysis of protein sequence alignments showed that EjPI has typical domains of M, I, K, and C, and includes a distinctive PI motif at the C-terminal region. Compared with asterids PI/GLO lineage, the K1 and K3 subdomains of EjPI both contain a single amino acid difference. Subcellular localization of EjPI was determined to be in the nucleus. Expression pattern analysis revealed that EjPI expressed not only in petals, filament, and anther in single-flower E. japonica, but also in petaloid sepals in double-flower E. japonica. Meanwhile, there were high correlation between EjPI transcript level and petaloid area within a sepal. Furthermore, 35S::EjPI transgenic wild-type Arabidopsis caused the homeotic transformation of the first whorl sepals into petaloid sepals. Ectopic expression of EjPI in transgenic pi-1 mutant Arabidopsis rescued normal petals and stamens. These results suggest expression pattern of EjPI is associated with the formation of petaloid sepal. Our study provides the potential application of EjPI for biotechnical engineering to create petaloid sepals or regulate floral organ identity in angiosperms.

更新日期：2020-01-17

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>