EGCG通过其在非小细胞肺癌细胞中的氧化作用调节CTR1的表达。,Journal of Cellular Physiology

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EGCG通过其在非小细胞肺癌细胞中的氧化作用调节CTR1的表达。
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-01-14 , DOI: 10.1002/jcp.29451
Aochang Chen ₁ , Pan Jiang ₁ , Falak Zeb ₁ , Xiaoyue Wu ₁ , Chuyue Xu ₁ , Lijun Chen ₁ , Qing Feng ₁

Affiliation

铜转运蛋白1（CTR1）在增加顺铂摄入中起重要作用。我们以前的研究表明，绿茶多酚（-）-表没食子儿茶素3-没食子酸酯（EGCG）上调了CTR1的表达，因此增强了卵巢癌和非小细胞肺癌（NSCLC）细胞的顺铂敏感性。在当前的非小细胞肺癌细胞研究中，我们发现了EGCG诱导CTR1的潜在氧化机制。我们发现EGCG可增加活性氧（ROS）的生成，而在存在ROS清除剂N的情况下-乙酰半胱氨酸（NAC），消除了ROS的产生。CTR1表达的变化与ROS水平一致。同时，EGCG通过ROS诱导ETR1 / 2上调，而通过ROS上调lncRNA核副斑点组装转录本1（NEAT1）上调。此外，在裸鼠异种移植模型中，EGCG处理可提高肿瘤组织中的ROS水平，CTR1和NEAT1的表达。同样，ERK1 / 2和p-ERK1 / 2也被抑制。综上所述，这些结果提示了EGCG介导ROS通过ERK1 / 2 / NEAT1信号通路调节CTR1表达的新机制，这为EGCG作为肺癌辅助治疗的天然药物提供了更多可能性。

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EGCG regulates CTR1 expression through its pro-oxidative property in non-small-cell lung cancer cells.

Copper transporter 1 (CTR1) plays an important role in increasing cisplatin intake. Our previous studies showed that CTR1 expression was upregulated by (−)‐epigallocatechin‐3‐gallate (EGCG), a green tea polyphenol, therefore enhanced cisplatin sensitivity in ovary cancer and non‐small‐cell lung cancer (NSCLC) cells. In the current study in the non‐small‐cell lung cancer cells, we uncovered a potential mechanism of EGCG‐induced CTR1 through its pro‐oxidative property. We found that EGCG increased reactive oxygen species (ROS) generation, while in the presence of ROS scavenger N‐acetyl‐cysteine (NAC), ROS production was eliminated. Changes of CTR1 expression were consistent with the ROS level. Simultaneously, EGCG downregulated ERK1/2 while upregulated lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) through ROS to induce CTR1 expression. Besides, in a nude mouse xenografts model, EGCG treatment raised ROS level, expression of CTR1 and NEAT1 in tumor tissue. Also, ERK1/2 and p‐ERK1/2 were suppressed as well. Taken together, these results suggested a novel mechanism that EGCG mediated ROS to regulate CTR1 expression through the ERK1/2/NEAT1 signaling pathway, which provided more possibilities for EGCG as a natural agent in adjuvant therapy of lung cancer.

更新日期：2020-01-14

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