We present a smartphone optosensing platform (SOP) using a digital versatile disc (DVD) diffraction grating for rapid in-field detecting neurotoxins. The smartphone holder and sample holder were 3D printed for the SOP. A DVD grating is demonstrated for the first time in a low-cost miniature spectrometer on the SOP to quantify the concentrations of neurotoxins. The SOP is capable of detecting optical absorbance spectra within the entire visible spectral range from 400 to 700 nm with the spectral resolution of 0.2521 nm/pixel. We demonstrated the performance of the DVD grating compared with a commercial transmission grating on the SOP and a conventional microplate reader. Paraoxon, as the selected neurotoxin model, is assayed by two types of cholinesterase (ChE) on our SOP, respectively. Integrating a DVD grating in the SOP allows quantification of paraoxon in the range from 5 nM to 25 μM with the detection limit of 2.9 nM. In addition to the low assessed detection limit at medically relevant concentrations, the performance of SOP with DVD gratings provides new avenues for a point-of-care toxin diagnosis with time and cost savings.

中文翻译：

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使用DVD光栅检测神经毒素的智能手机光电传感平台

我们提出了一种使用数字多功能光盘（DVD）衍射光栅的智能手机光传感平台（SOP），用于快速现场检测神经毒素。智能手机支架和样品支架均经过3D打印以用于SOP。在SOP上的低成本微型光谱仪中首次展示了DVD光栅，以量化神经毒素的浓度。SOP能够以0.2521 nm /像素的光谱分辨率检测从400到700 nm的整个可见光谱范围内的光吸收光谱。与SOP上的商用透射光栅和传统的酶标仪相比，我们展示了DVD光栅的性能。作为选择的神经毒素模型，对氧磷是通过我们SOP上的两种类型的胆碱酯酶（ChE）进行分析的。在SOP中集成DVD光栅可以在5 nM至25μM的范围内对氧磷进行定量，检测极限为2.9 nM。除了在医学上相关浓度下的低检测限外，具有DVD光栅的SOP的性能还提供了一种即时诊断毒素的新途径，可以节省时间和成本。