基于AIEgens的双光子荧光生物成像有机点,Small

当前位置： X-MOL 学术 › Small › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

基于AIEgens的双光子荧光生物成像有机点
Small ( IF 13.0 ) Pub Date : 2016-06-30 , DOI: 10.1002/smll.201600872
Xiaoding Lou ₁ , Zujin Zhao ₂ , Ben Zhong Tang _{2,

3,

4}

Affiliation

就高光稳定性，低光损伤，高时空分辨率而言，双光子荧光成像技术是一种强大的生物分析方法。最近，由于有机发光芯和聚合物基体组成的荧光有机点具有高且可调节的荧光，良好的生物相容性，强的耐光漂白性和多种表面功能等优点，因此成为用于双光子荧光成像的有前途的造影剂。发射核对于有机点获得高亮度至关重要，但是许多传统的生色团在形成聚集体时通常会遇到严重的荧光猝灭问题。为了解决这个问题，具有聚集诱导发射（AIE）的氟气可以在聚集体中强烈发出荧光，因此成为荧光有机点的理想候选者。此外，只需增加AIE氟（AIEgen）的负载量，即可轻松改善点的两光子吸收特性。因此，基于AIEgens的有机点在双光子荧光体外细胞成像以及小鼠皮肤，肌肉，大脑和颅骨的体内血管结构可视化中均表现出出色的性能。鉴于这一重要研究领域的快速发展，在这里，我们重点介绍具有AIEgen聚集体发射核的代表性荧光有机点，并讨论它们在生物成像应用中的巨大潜力。基于AIEgens的有机点在双光子荧光体外细胞成像以及小鼠皮肤，肌肉，大脑和颅骨的体内血管结构可视化中均表现出出色的性能。鉴于这一重要研究领域的快速发展，在这里，我们重点介绍具有AIEgen聚集体发射核的代表性荧光有机点，并讨论它们在生物成像应用中的巨大潜力。基于AIEgens的有机点在双光子荧光体外细胞成像以及小鼠皮肤，肌肉，大脑和颅骨的体内血管结构可视化中均表现出出色的性能。鉴于这一重要研究领域的快速发展，在这里，我们重点介绍具有AIEgen聚集体发射核的代表性荧光有机点，并讨论它们在生物成像应用中的巨大潜力。

"点击查看英文标题和摘要"

Organic Dots Based on AIEgens for Two‐Photon Fluorescence Bioimaging

Two‐photon fluorescence imaging technique is a powerful bioanalytical approach in terms of high photostability, low photodamage, high spatiotemporal resolution. Recently, fluorescent organic dots comprised of organic emissive cores and a polymeric matrix are emerging as promising contrast reagents for two‐photon fluorescence imaging, owing to their numerous merits of high and tunable fluorescence, good biocompatibility, strong photobleaching resistance, and multiple surface functionality. The emissive core is crucial for organic dots to get high brightness but many conventional chromophores often encounter a severe problem of fluorescence quenching when they form aggregates. To solve this problem, fluorogens featuring aggregation‐induced emission (AIE) can fluoresce strongly in aggregates, and thus become ideal candidates for fluorescent organic dots. In addition, two‐photon absorption property of the dots can be readily improved by just increase loading contents of AIE fluorogen (AIEgen). Hence, organic dots based on AIEgens have exhibited excellent performances in two‐photon fluorescence in vitro cellular imaging, and in vivo vascular architecture visualization of mouse skin, muscle, brain and skull bone. In view of the rapid advances in this important research field, here, we highlight representative fluorescent organic dots with an emissive core of AIEgen aggregate, and discuss their great potential in bioimaging applications.

更新日期：2016-06-30

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南

全部期刊列表>>