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Immobilized Glucose Oxidase on Boronic Acid-Functionalized Hierarchically Porous MOF as an Integrated Nanozyme for One-Step Glucose Detection
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-03-10 , DOI: 10.1021/acssuschemeng.9b07631 Zhenghong Zhao 1 , Yaojing Huang 1 , Wenren Liu 1 , Fanggui Ye 1 , Shulin Zhao 1
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-03-10 , DOI: 10.1021/acssuschemeng.9b07631 Zhenghong Zhao 1 , Yaojing Huang 1 , Wenren Liu 1 , Fanggui Ye 1 , Shulin Zhao 1
Affiliation
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Metal–organic frameworks (MOFs) with uniform porosity and large surface areas can function as carriers of immobilized enzymes. However, drawbacks including long response times or enzyme leakage have hindered their applicability. In this study, a boronic acid-functionalized hierarchically porous MIL-88B (HP-MIL-88B-BA) was prepared as an efficient immobilization matrix for glucose oxidase (GOx). HP-MIL-88B-BA features a hierarchical, porous structure with sufficient recognition sites that facilitate GOx immobilization and prevent enzyme leakage. This hierarchical porosity increased the substrate mass transfer efficiency, thereby reducing the response time. Moreover, HP-MIL-88B-BA can be used as a horseradish peroxidase mimic. In this manner, an integrated nanozyme was constructed for the rapid one-step detection of glucose. [email protected] exhibited a rapid response to glucose (10 min) and displayed a good linear relationship from 2 to 100 μM with a detection limit of 0.98 μM. This novel methodology provides a straightforward, rapid, and efficient strategy for integrated nanozyme synthesis for biosensing purposes.
中文翻译：
固定在硼酸官能化多孔MOF上的固定化葡萄糖氧化酶，作为用于一步法葡萄糖检测的集成纳米酶
具有均匀孔隙率和大表面积的金属有机骨架（MOF）可以充当固定化酶的载体。但是，包括长响应时间或酶泄漏的缺点阻碍了它们的适用性。在这项研究中，硼酸官能化的分级多孔MIL-88B（HP-MIL-88B-BA）被制备为葡萄糖氧化酶（GOx）的有效固定基质。HP-MIL-88B-BA具有分层的多孔结构，具有足够的识别位点，可促进GOx固定并防止酶泄漏。这种分级的孔隙率提高了基板的传质效率，从而缩短了响应时间。此外，HP-MIL-88B-BA可用作辣根过氧化物酶模拟物。以这种方式，构建了集成的纳米酶，用于快速一步检测葡萄糖。[电子邮件保护]对葡萄糖表现出快速响应（10分钟），并显示2至100μM的良好线性关系，检出限为0.98μM。这种新颖的方法为生物传感目的提供了一种集成纳米酶合成的直接，快速，有效的策略。
更新日期：2020-03-10
中文翻译：
固定在硼酸官能化多孔MOF上的固定化葡萄糖氧化酶，作为用于一步法葡萄糖检测的集成纳米酶
具有均匀孔隙率和大表面积的金属有机骨架（MOF）可以充当固定化酶的载体。但是，包括长响应时间或酶泄漏的缺点阻碍了它们的适用性。在这项研究中，硼酸官能化的分级多孔MIL-88B（HP-MIL-88B-BA）被制备为葡萄糖氧化酶（GOx）的有效固定基质。HP-MIL-88B-BA具有分层的多孔结构，具有足够的识别位点，可促进GOx固定并防止酶泄漏。这种分级的孔隙率提高了基板的传质效率，从而缩短了响应时间。此外，HP-MIL-88B-BA可用作辣根过氧化物酶模拟物。以这种方式，构建了集成的纳米酶，用于快速一步检测葡萄糖。[电子邮件保护]对葡萄糖表现出快速响应（10分钟），并显示2至100μM的良好线性关系，检出限为0.98μM。这种新颖的方法为生物传感目的提供了一种集成纳米酶合成的直接，快速，有效的策略。
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