This research focuses on extraction of medicinal plant Launaea acanthodes (L. acanthodes) by using supercritical carbon dioxide (SCCO₂) and hydro-distillation (HD) methods. The influence of pressure, temperature, flow rate and volume of co-solvent on the extraction yield of L. acanthodes was investigated by response surface methodology (RSM) and artificial neural network (ANN). The error values of RSM (R² = 0.9871, MSE = 0.000016 and AARD = 2.395) and ANN (R² = 0.9968, MSE = 0.0000057 and AARD = 1.301) were calculated. The results exhibited that both models have the ability to predict the experimental data, but ANN was more reliable than the RSM. The maximum extraction yield was estimated by RSM to be 1.0250% under the operational conditions of pressure 240 bar, temperature 328 K, flow rate 4.06 g/min and co-solvent 663 μl; whereas the optimum extraction yield estimated by hybrid ANN-GA method was 1.0238% applying the operational conditions of pressure 240 bar, temperature 328 K, flow rate 3.98 g/min and co-solvent 650 μl. The maximum yield (1.0238%) obtained at the optimal conditions was more than that achieved by hydro-distillation method (0.7530%). The extracted components obtained from both methods were analyzed by GC/MS and GC-FID. The main compounds found in the extracts were γ-Phenylbutyric acid (9.29, 9.19%), Geranyl acetone (7.67, 7.87%), n-Hexadecane (7.34, 6.26%), (Z)-3-Hexen-1-yl-benzoate (7.10, 6.91%), n-Dodecanal (5.87, 6.56%), (2Z,6E)-Farnesol (5.65, 4.34%) and Benzoic acid n-hexyl ester (5.13, 4.51%) and β-Ionone (4.55, 4.58%) for SCCO₂ and HD methods, respectively. To the best of our knowledge, this is the first report on the extraction of essential oil from L. acanthodes.

这项研究的重点是通过使用超临界二氧化碳（SC CO ₂）和加氢蒸馏（HD）方法提取药用植物Launaea acanthodes（L. acanthodes）。通过响应面法（RSM）和人工神经网络（ANN）研究了压力，温度，流速和助溶剂体积对刺槐提取率的影响。RSM（R ²  = 0.9871，MSE = 0.000016和AARD = 2.395）和ANN（R ² = 0.9968，MSE = 0.0000057和AARD = 1.301）。结果表明，这两种模型都具有预测实验数据的能力，但是ANN比RSM更可靠。通过RSM在压力为240 bar，温度为328 K，流速为4.06 g / min和助溶剂为663μl的操作条件下估计最大提取产率为1.0250％；而在压力为240 bar，温度为328 K，流速为3.98 g / min和助溶剂为650μl的操作条件下，通过混合ANN-GA方法估算的最佳提取率为1.0238％。在最佳条件下获得的最大收率（1.0238％）比通过水蒸馏法获得的最大收率（0.7530％）高。通过GC / MS和GC-FID分析从两种方法获得的提取成分。正十六烷（7.34，6.26％），（Z）-3-己基-1-基苯甲酸酯（7.10，6.91 ％），正十二烷（5.87，6.56％），（2Z，6E）-法尼索尔（5.65，对于SC CO ₂和HD方法，分别为4.34％）和苯甲酸正己酯（5.13，4.51％）和β-紫罗兰酮（4.55，4.58％）。据我们所知，这是关于从刺五加中提取精油的第一份报告。