2-Vinyl-4,6-diamino-1,3,5-triazine (VDAT) polymers have demonstrated functionality of adsorbing water-soluble molecules via stable hydrogen bonding in aqueous environment. However, traditional methods for the polymer particle synthesis face challenges due to the poor solubility of VDAT. In this study, a semi-continuous precipitation polymerization was developed to synthesize VDAT polymer nanoparticles in aqueous phase. By continuously feeding VDAT to hot water in the presence of a cationic free radical initiator, the dissolved VDAT underwent precipitation polymerization and its concentration was kept below the saturation limit while the fragments of ionic initiator provided electrostatic repulsion for particle stabilization. VDAT feeding rate and the monomer to initiator ratio throughout the polymerization were the key factors to achieve good particle morphology and narrow size distribution. The synthesized nanoparticles quickly adsorbed bovine hemoglobin in water. The adsorption isotherm study and theoretical estimation suggested a monolayer adsorption. The adsorption efficiency varied significantly with both pH and ionic strength which were controlled to manipulate physiochemical characteristics of both the nanoparticles and the protein macromolecules. The adsorption results suggested an important role of hydrogen bonding and its interactions with electrostatic attraction/repulsion and hydrophobic affinity.

2-乙烯基-4,6-二氨基-1,3,5-三嗪（VDAT）聚合物具有在水性环境中通过稳定的氢键吸附水溶性分子的功能。然而，由于VDAT的溶解性差，用于聚合物颗粒合成的传统方法面临挑战。在这项研究中，开发了一种半连续沉淀聚合以在水相中合成VDAT聚合物纳米颗粒。通过在阳离子自由基引发剂的存在下将VDAT连续供入热水中，溶解的VDAT进行了沉淀聚合，其浓度保持在饱和极限以下，而离子引发剂的碎片为颗粒稳定提供了静电排斥力。在整个聚合过程中，VDAT进料速率和单体与引发剂的比例是获得良好的颗粒形态和窄尺寸分布的关键因素。合成的纳米颗粒在水中快速吸附牛血红蛋白。吸附等温线研究和理论估计表明单层吸附。吸附效率随pH和离子强度的变化而显着变化，而pH和离子强度均受到控制以控制纳米颗粒和蛋白质大分子的理化特性。吸附结果表明氢键及其与静电吸引/排斥和疏水亲和力的相互作用具有重要作用。吸附等温线研究和理论估计表明单层吸附。吸附效率随pH和离子强度的变化而显着变化，而pH和离子强度均受到控制以控制纳米颗粒和蛋白质大分子的理化特性。吸附结果表明氢键及其与静电吸引/排斥和疏水亲和力的相互作用具有重要作用。吸附等温线研究和理论估计表明单层吸附。吸附效率随pH和离子强度的变化而显着变化，而pH和离子强度均受控制以控制纳米颗粒和蛋白质大分子的理化特性。吸附结果表明氢键及其与静电吸引/排斥和疏水亲和力的相互作用具有重要作用。