Abstract The conversion of ricinoleic acid from renewable sources to long-chain α,ω-dicarboxylic acids or w-hydroxyl carboxylic acids by microbial processes is constrained by toxicity issues. Here, we demonstrate the possible role of Corynebacterium glutamicum as a new microbial strategy for the biotransformation of fatty acids. The established strain Escherichia coli failed to grow at 5 mM n-heptanoic acid, while the specific growth rate of C. glutamicum declined by 28%. We partially constructed a previously designed multistep biocatalytic pathway in C. glutamicum, and confirmed that the C. glutamicum biocatalyst successfully converted ricinoleic acid to undec-9-enoic acid, heptyl ester via 12-keto-oleic acid. We investigated the effects of cultivation and reaction temperatures, and the type and concentration of non-ionic detergent on recombinant C. glutamicum whole-cell bioconversion. At a cultivation temperature of 30 °C and a reaction temperature of 35 °C, and in the presence of 0.09 g/L Triton X-100, the whole-cell C. glutamicum biocatalyst produced 0.8 mM undec-9-enoic acid, heptyl ester from 1.9 mM 12-ketooleic acid. It also generated 0.7 mM undec-9-enoic acid, heptyl ester from 5.5 mM ricinoleic acid. This is the first report of undec-9-enoic acid, heptyl ester production using a recombinant C. glutamicum-based biocatalyst.

中文翻译：

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使用基于重组谷氨酸棒杆菌的全细胞生物催化剂从可再生脂肪酸中微生物合成十一碳烯酸、庚酯

摘要 通过微生物过程将可再生来源的蓖麻油酸转化为长链 α,ω-二羧酸或 w-羟基羧酸受到毒性问题的限制。在这里，我们证明了谷氨酸棒杆菌作为脂肪酸生物转化的新微生物策略的可能作用。已建立的大肠杆菌菌株在 5 mM 正庚酸下无法生长，而谷氨酸棒杆菌的比生长率下降了 28%。我们在谷氨酸棒杆菌中部分构建了先前设计的多步生物催化途径，并证实谷氨酸棒杆菌生物催化剂通过 12-酮油酸成功地将蓖麻油酸转化为十一碳烯酸庚酯。我们研究了培养和反应温度的影响，以及非离子去污剂的类型和浓度对重组谷氨酸棒杆菌全细胞生物转化的影响。在 30 °C 的培养温度和 35 °C 的反应温度下，在 0.09 g/L Triton X-100 的存在下，全细胞谷氨酸棒杆菌生物催化剂产生了 0.8 mM undec-9-enoic acid、heptyl来自 1.9 mM 12-酮油酸的酯。它还从 5.5 mM 蓖麻油酸生成 0.7 mM undec-9-烯酸庚酯。这是使用重组谷氨酸棒杆菌生物催化剂生产十一碳烯酸庚酯的第一份报告。它还从 5.5 mM 蓖麻油酸生成 0.7 mM undec-9-烯酸庚酯。这是使用重组谷氨酸棒杆菌生物催化剂生产十一碳烯酸庚酯的第一份报告。它还从 5.5 mM 蓖麻油酸生成 0.7 mM undec-9-烯酸庚酯。这是使用重组谷氨酸棒杆菌生物催化剂生产十一碳烯酸庚酯的第一份报告。