Abstract Arachidonoyl ethanolamide (AEA) is a bioactive lipid that occurs naturally in animal and plant tissues. In this study, arachidonic acid (ARA) was enriched first from a type of microbial oil by a two-step purification, and the purified ARA concentrate was then used for the enzymatic preparation of AEA by amidation between ARA and ethanolamine. The purity of initial ARA increased from 44.4% to 85.1% after urea complexation and acetonitrile extraction. The purified ARA was subsequently used for lipase-catalyzed synthesis of AEA. The results showed that under optimized conditions (5 mmol purified ARA product, 5.5 mmol ethanolamine, 3 mL hexane, 10% Lipozyme 435, 70 °C for 2 h), fatty acid ethanolamide was obtained with 95.6% yield. The current method for making AEA by the enzymatic amidation of ethanolamine with ARA from fungal oil is effective, practical and economically feasible.

中文翻译：

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微生物油提纯花生四烯酸酶酰胺化制备花生四烯酰乙醇酰胺

摘要 花生四烯酰乙醇酰胺 (AEA) 是一种天然存在于动植物组织中的生物活性脂质。在这项研究中，花生四烯酸 (ARA) 首先通过两步纯化从一类微生物油中富集，然后将纯化的 ARA 浓缩物用于通过 ARA 和乙醇胺之间的酰胺化反应制备 AEA。尿素络合和乙腈提取后，初始ARA的纯度从44.4%提高到85.1%。随后将纯化的 ARA 用于脂肪酶催化的 AEA 合成。结果表明，在优化条件下（5 mmol纯化的ARA产物、5.5 mmol乙醇胺、3 mL己烷、10% Lipozyme 435、70°C 2 h），以95.6%的产率获得了脂肪酸乙醇酰胺。