从 CO2 生产聚（3-羟基丁酸酯）（PHB）：模型开发和工艺优化,Biochemical Engineering Journal

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从 CO2 生产聚（3-羟基丁酸酯）（PHB）：模型开发和工艺优化
Biochemical Engineering Journal ( IF 3.7 ) Pub Date : 2015-06-01 , DOI: 10.1016/j.bej.2015.02.031
Md. Salatul Islam Mozumder , Linsey Garcia-Gonzalez , Heleen De Wever , Eveline I.P. Volcke

摘要直接从二氧化碳 (CO 2 ) 生物合成聚 (3-羟基丁酸酯) (PHB) 是不可再生的石油基聚合物生产的可持续替代方案。CO 2 的转化意味着温室气体排放的减少。诸如Cupriavidus necator 之类的氢氧化细菌能够使用CO 2 作为碳源，即通过自养转化来储存PHB。在这项研究中，建立了基于质量平衡的数学模型来描述自养 PHB 生产。该模型考虑了生物质生长和 PHB 形成的化学计量和动力学，以及从气相到液体发酵液的物理转移。开发的模型基于来自文献的独立实验数据集进行校准和验证，这些数据集是为 C. necator 获得的。获得的模拟结果准确地描述了自养生物量生长和 PHB 生产的动态。通过情景分析研究了氧气 (O 2 ) 和/或氮气应力条件以及气体混合物组成对 O 2 和氢气 (H 2 ) 的影响。作为主要结果，与氮胁迫条件相比，在氧胁迫条件下获得了更高的最大 PHB 浓度。在气体混合物中的高 O 2 分数下，这将导致 H 2 在 O 2 限制之前受到限制，因此可以通过施加氮气压力来增加 PHB 的生产。通过比较连续搅拌罐式反应器 (CSTR) 和气升式发酵罐来评估反应器类型的影响。

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Poly(3-hydroxybutyrate) (PHB) production from CO2: Model development and process optimization

Abstract The biosynthesis of poly(3-hydroxybutyrate) (PHB) directly from carbon dioxide (CO 2 ), is a sustainable alternative for non-renewable, petroleum-based polymer production. The conversion of CO 2 implies a reduction of greenhouse gas emissions. Hydrogen oxidizing bacteria such as Cupriavidus necator have the ability to store PHB using CO 2 as a carbon source, i.e., through an autotrophic conversion. In this study, a mathematical model based on mass balances was set up to describe autotrophic PHB production. The model takes into account the stoichiometry and kinetics of biomass growth and PHB formation as well as physical transfer from the gas phase to the liquid fermentation broth. The developed model was calibrated and validated based on independent experimental datasets from literature, obtained for C. necator . The obtained simulation results accurately described the dynamics of autotrophic biomass growth and PHB production. The effect of oxygen (O 2 ) and/or nitrogen stress conditions, as well as of the gas mixture composition in terms of O 2 and hydrogen (H 2 ) was investigated through scenario analysis. As major outcome, a higher maximum PHB concentration was obtained under oxygen stress conditions compared to nitrogen stress conditions. At high O 2 fractions in the gas mixture, which would result in H 2 limitation before O 2 limitation, PHB production can be increased by applying nitrogen stress. The effect of the reactor type was assessed through comparing a continuous stirred tank reactor (CSTR) with an air-lift fermentor. The developed model forms the basis for future design with minimum experimentation of suitable control strategy aiming at a high PHB production.

更新日期：2015-06-01

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