A new rhodamine B derivative, namely, N¹-(benzo[d]thiazol-2-yl)-N⁴-(2-(3′,6′-bis (diethylamino)-3-oxospiro[isoindoline-1,9′-xanthen]-2-yl)ethyl)malea-mide (RDBSF), was designed, synthesized, and structurally characterized to develop a new chemosensor. UV–vis absorption and fluorescence spectroscopic studies show that RDBSF exhibits high sensitivity and selectivity toward Fe³⁺ in the presence of many other metal cations in a Tris-HCl solution (1 mM, pH = 7.4) containing 30% MeCN by forming a 1:1 complex with Fe³⁺. The binding association constant (K_a) of RDBSF for Fe³⁺ was estimated as 1.52 × 10⁴ M⁻¹ in the solution. The detection limit of Fe³⁺ by RDBSF was further determined as 11.6 nM. Intracellular imaging applications demonstrated that RDBSF can be used as a fluorescent probe for the detection of Fe³⁺ in HepG-2 cells.

一种新的若丹明B衍生物，即N ^1-（苯并[d]噻唑-2-基）-N ^4-（2-（3'，6'-双（二乙基氨基）-3-氧代螺[异吲哚啉-1,9]设计，合成了'-'xanthen] -2-基）乙基）马来酰亚胺（RDBSF），并对其结构进行了表征，以开发新型的化学传感器。紫外可见吸收和荧光光谱研究表明，在含有30％MeCN的Tris-HCl溶液（1 mM，pH = 7.4）中，许多其他金属阳离子存在时，RDBSF对Fe ³⁺表现出高灵敏度和选择性，形成1 ：1与Fe ^{3+的配合物}。RDBSF与Fe ³⁺的结合缔合常数（K _a）估计为1.52×10溶液中为⁴  M ^-1。Fe的检测限³⁺由RDBSF进一步确定为11.6纳米。细胞内成像应用表明，RDBSF可用作检测HepG-2细胞中Fe ³⁺的荧光探针。