It is significant to develop probes for rapid, selective, and sensitive detection of the highly toxic hydrazine in both environmental and biological science. In this work, under mild condition, a novel colorimetric and ratiometric fluorescent probe (probe 1) was synthesized. Probe 1 with a chemoselective reaction mediated by hydrazine in which the diethyl malonate moiety was transformed into an aldehyde group exhibited high sensitivity and good selectivity in hydrazine recognition. Probe 1 can quantitatively detect hydrazine in concentration range from 0 to 120 μM with the LOD of 1.6 μM. Further, it displayed good selectivity and anti-interference over many species including some nucleophilic species, anions and metal ions. The ratiometric fluorescence change of this probe upon addition of hydrazine in living cells has been successfully demonstrated. Furthermore, the probe displays low dark toxicity under physiological temperature.

开发用于快速，选择性和灵敏地检测环境和生物科学中的剧毒肼的探针具有重要意义。在这项工作中，在温和的条件下，合成了一种新型的比色和比例荧光探针（探针1）。具有由肼介导的化学选择性反应的探针1，其中丙二酸二乙酯部分被转化成醛基，在肼识别中表现出高灵敏度和良好的选择性。探针1可以定量检测0至120μM范围内的肼，LOD为1.6μM。此外，它对包括某些亲核物种，阴离子和金属离子在内的许多物种都表现出良好的选择性和抗干扰性。已经成功地证明了在活细胞中加入肼后，该探针的比例荧光变化。此外，该探针在生理温度下显示出低的暗毒性。