The earthworm Lumbricus rubellus (Hoffmeister, 1843) is a terrestrial pollution sentinel. Enzyme activity and transcription of phase II detoxification superfamily glutathione transferases (GST) is known to respond in earthworms after soil toxin exposure, suggesting GST as a candidate molecular-based pollution biomarker. This study combined sub-proteomics, bioinformatics and biochemical assay to characterise the L. rubellus GST complement as pre-requisite to initialise assessment of the applicability of GST as a biomarker. L. rubellus possesses a range of GSTs related to known classes, with evidence of tissue-specific synthesis. Two affinity-purified GSTs dominating GST protein synthesis (Sigma and Pi class) were cloned, expressed and characterised for enzyme activity with various substrates. Electrospray ionisation mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS) following SDS-PAGE were superior in retaining subunit stability relative to two-dimensional gel electrophoresis (2-DE). This study provides greater understanding of Phase II detoxification GST superfamily status of an important environmental pollution sentinel organism.

蚯蚓地龙rubellus的（霍夫迈斯特，1843）是地面的污染定点。已知II期解毒超家族谷胱甘肽转移酶（GST）的酶活性和转录在土壤毒素暴露后对worm有反应，这表明GST是一种候选的基于分子的污染生物标志物。这项研究结合了蛋白质组学，生物信息学和生化分析，以表征风疹乳杆菌GST补体的特征，这是初始化评估GST作为生物标记物的适用性的前提。风信子拥有与已知类别相关的一系列GST，并具有组织特异性合成的证据。克隆，表达并表征了两种以GST蛋白合成为主的亲和纯化GST（Sigma和Pi类），并用各种底物表征了酶的活性。SDS-PAGE后的电喷雾电离质谱（ESI-MS）和串联质谱（MS / MS）在保留亚基稳定性方面优于二维凝胶电泳（2-DE）。这项研究为重要环境污染定点生物的II期解毒GST超家族状态提供了更深入的了解。