It is highly demanded and challenging to develop small-molecular fluorescent sensors for the ratiometric detection of pH fluctuation. A ratiometric fluorescent pH sensor was constructed by integrating a pH-sensitive fluorophore with a pH-insensitive hemicyanine group. Its linear and reversible ratiometric fluorescent response from pH 7.0 to 8.8 and 6.0 to 9.0 respectively makes this sensor suitable for the practical tracking of pH fluctuation in live cells. With this sensor, stimulated pH fluctuation has been successfully tracked in a ratiometric manner via fluorescence imaging with low dark toxicity.

中文翻译：

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用于pH波动的比例荧光传感器及其在低暗毒性的活细胞中的应用

迫切需要开发小分子荧光传感器以用于pH波动的比例检测。通过将pH敏感的荧光团与pH不敏感的半花菁基团整合在一起，构建了比率式荧光pH传感器。它的线性和可逆比例荧光响应分别从pH 7.0至8.8和6.0至9.0，使该传感器适合实际跟踪活细胞中的pH波动。使用该传感器，已通过具有低暗毒性的荧光成像以比例方式成功跟踪了受刺激的pH波动。