Abstract Porphyrins modified inorganic semiconductors have been paid more attention as a platform in nanomaterials, due to the fast electronic transfer from synergistic effect. Meso-tetrakis(4-chlorophenyl)porphyrin functionalized CuFe2O4/SiO2 nanocomposites (TClPP-CuFe2O4/SiO2) were prepared by a simple method and found to exhibit the higher peroxidase-like activity, compared with that of CuFe2O4/SiO2 without porphyrin. The TClPP-CuFe2O4/SiO2 nanocomposites can effectively catalyze the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) by H2O2 to give a blue product (oxTMB) only in 3 min, which can be conveniently distinguished by the naked eye. Notably, the optimal response temperature was 25 °C (room temperature), lower than that of most of nanomaterials as peroxidase mimics, which is in favor of practical application. Based on the fast response as well as the peroxidase activity of TClPP-CuFe2O4/SiO2, a simple, sensitive, selective, colorimetric method was proposed for detection of H2O2 over a range of 8 to 80 μM with a detection limit of 1.02 μM. Furthermore, the system including TClPP-CuFe2O4/SiO2, glucose oxidase(GOx) and the substrate (TMB), provided a colorimetric sensor for glucose in the range of 5 - 100 μM with a detection Limit of 3.62 μM.

中文翻译：

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具有增强的类过氧化物酶活性的中四（4-氯苯基）卟啉功能化的 CuFe2O4/SiO2 纳米复合材料可方便地用于室温下的视觉生物传感

摘要 卟啉修饰的无机半导体作为纳米材料的平台，由于协同效应的快速电子转移而受到越来越多的关注。通过简单的方法制备了内消旋四（4-氯苯基）卟啉功能化的 CuFe2O4/SiO2 纳米复合材料（TClPP-CuFe2O4/SiO2），发现与不含卟啉的 CuFe2O4/SiO2 相比，它表现出更高的类过氧化物酶活性。TClPP-CuFe2O4/SiO2纳米复合材料可以有效地催化过氧化物酶底物3,3',5,5'-四甲基联苯胺（TMB）被H2O2氧化，仅在3分钟内得到蓝色产物（oxTMB），可以方便地进行区分用肉眼。值得注意的是，最佳响应温度为25°C（室温），低于大多数作为过氧化物酶模拟物的纳米材料的响应温度，有利于实际应用。基于 TClPP-CuFe2O4/SiO2 的快速响应和过氧化物酶活性，提出了一种简单、灵敏、选择性的比色法，用于检测 8 至 80 μM 范围内的 H2O2，检测限为 1.02 μM。此外，该系统包括TClPP-CuFe2O4/SiO2、葡萄糖氧化酶(GOx)和底物(TMB)，提供了5-100 μM范围内葡萄糖的比色传感器，检测限为3.62 μM。