Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Diversity and function of multicopper oxidase genes in the stinkbug Plautia stali.
Scientific Reports ( IF 3.8 ) Pub Date : 2020-02-26 , DOI: 10.1038/s41598-020-60340-8
Yudai Nishide 1 , Daisuke Kageyama 1 , Masatsugu Hatakeyama 1 , Kakeru Yokoi 1 , Akiya Jouraku 1 , Hiromitsu Tanaka 1 , Ryuichi Koga 2 , Ryo Futahashi 2 , Takema Fukatsu 2, 3, 4
Scientific Reports ( IF 3.8 ) Pub Date : 2020-02-26 , DOI: 10.1038/s41598-020-60340-8
Yudai Nishide 1 , Daisuke Kageyama 1 , Masatsugu Hatakeyama 1 , Kakeru Yokoi 1 , Akiya Jouraku 1 , Hiromitsu Tanaka 1 , Ryuichi Koga 2 , Ryo Futahashi 2 , Takema Fukatsu 2, 3, 4
Affiliation
![]() |
Multicopper oxidase (MCO) genes comprise multigene families in bacteria, fungi, plants and animals. Two families of MCO genes, MCO1 (laccase1) and MCO2 (laccase2), are conserved among diverse insects and relatively well-characterized, whereas additional MCO genes, whose biological functions have been poorly understood, are also found in some insects. Previous studies reported that MCO1 participates in gut immunity and MCO2 plays important roles in cuticle sclerotization and pigmentation of insects. In mosquitoes, MCO2 was reported to be involved in eggshell sclerotization and pigmentation, on the ground that knockdown of MCO2 caused deformity and fragility of the eggshell. Here we identified a total of 7 MCO genes, including PsMCO1 and PsMCO2, and investigated their expression and function in the brown-winged green stinkbug Plautia stali. RNA interference (RNAi) knockdown of MCO genes by injecting double-stranded RNA (dsRNA) into nymphs revealed that MCO2, but not the other 6 MCOs, is required for cuticle sclerotization and pigmentation, and also for survival of P. stali. Trans-generational knockdown of MCO2 by injecting dsRNA into adult females (maternal RNAi) resulted in the production of unhatched eggs despite the absence of deformity or fragility of the eggshell. These results suggested that MCO2 plays an important role in sclerotization and pigmentation of the cuticle but not in eggshell integrity in P. stali. Maternal RNAi of any of the other 6 MCO genes and 3 tyrosinase genes affected neither survival nor eggshell integrity of P. stali. Contrary to the observations in the red flour beetle and the brown rice planthopper, RNAi knockdown of MCO6 (MCORP; Multicopper oxidase related protein) exhibited no lethal effects on P. stali. Taken together, our findings provide insight into the functional diversity and commonality of MCOs across hemipteran and other insect groups.
中文翻译:
臭鼬Platutia stali中多铜氧化酶基因的多样性和功能。
多铜氧化酶(MCO)基因包含细菌,真菌,植物和动物中的多基因家族。MCO基因的两个家族,MCO1(laccase1)和MCO2(laccase2),在各种昆虫中都很保守并且具有相对较好的特征,而在某些昆虫中还发现了生物学功能尚未被很好理解的其他MCO基因。先前的研究报道,MCO1参与肠道免疫,MCO2在表皮硬化和昆虫色素沉着中起重要作用。据报道,在蚊子中,MCO2参与了蛋壳的硬化和色素沉着,原因是MCO2的敲低会导致蛋壳的变形和脆性。在这里,我们鉴定了总共7个MCO基因,包括PsMCO1和PsMCO2,并研究了它们在棕翅绿色臭椿Plautia stali中的表达和功能。通过向若虫中注射双链RNA(dsRNA)来抑制MCO基因的RNA干扰(RNAi)揭示,角质层硬化和色素沉着以及鳞茎毕生菌的生存都需要MCO2,但不需要其他6个MCO。通过将dsRNA注入成年雌性动物(母体RNAi)来进行跨代MCO2抑制,尽管卵壳没有畸形或脆弱,但仍产生了未孵化的卵。这些结果表明,MCO 2在角质层的表皮的硬化和色素沉着中起着重要作用,但在P. stali的蛋壳完整性中不起作用。其他6个MCO基因和3个酪氨酸酶基因中任何一个的母体RNAi均不影响St. stali的存活或卵壳完整性。与在红色面粉甲虫和糙米飞虱中观察到的相反,MCO6(MCORP; Multicopper氧化酶相关蛋白)对P. stali没有致死作用。综上所述,我们的发现为跨半足动物和其他昆虫群体的MCO的功能多样性和共性提供了见解。
更新日期:2020-02-26
中文翻译:

臭鼬Platutia stali中多铜氧化酶基因的多样性和功能。
多铜氧化酶(MCO)基因包含细菌,真菌,植物和动物中的多基因家族。MCO基因的两个家族,MCO1(laccase1)和MCO2(laccase2),在各种昆虫中都很保守并且具有相对较好的特征,而在某些昆虫中还发现了生物学功能尚未被很好理解的其他MCO基因。先前的研究报道,MCO1参与肠道免疫,MCO2在表皮硬化和昆虫色素沉着中起重要作用。据报道,在蚊子中,MCO2参与了蛋壳的硬化和色素沉着,原因是MCO2的敲低会导致蛋壳的变形和脆性。在这里,我们鉴定了总共7个MCO基因,包括PsMCO1和PsMCO2,并研究了它们在棕翅绿色臭椿Plautia stali中的表达和功能。通过向若虫中注射双链RNA(dsRNA)来抑制MCO基因的RNA干扰(RNAi)揭示,角质层硬化和色素沉着以及鳞茎毕生菌的生存都需要MCO2,但不需要其他6个MCO。通过将dsRNA注入成年雌性动物(母体RNAi)来进行跨代MCO2抑制,尽管卵壳没有畸形或脆弱,但仍产生了未孵化的卵。这些结果表明,MCO 2在角质层的表皮的硬化和色素沉着中起着重要作用,但在P. stali的蛋壳完整性中不起作用。其他6个MCO基因和3个酪氨酸酶基因中任何一个的母体RNAi均不影响St. stali的存活或卵壳完整性。与在红色面粉甲虫和糙米飞虱中观察到的相反,MCO6(MCORP; Multicopper氧化酶相关蛋白)对P. stali没有致死作用。综上所述,我们的发现为跨半足动物和其他昆虫群体的MCO的功能多样性和共性提供了见解。