Lipid Bicelle Micropatterning Using Chemical Lift-Off Lithography.,ACS Applied Materials & Interfaces

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Lipid Bicelle Micropatterning Using Chemical Lift-Off Lithography.
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2020-02-24 , DOI: 10.1021/acsami.9b20617
Jason N Belling _{1,

2} , Kevin M Cheung _{1,

2} , Joshua A Jackman _{3,

4} , Tun Naw Sut ₅ , Matthew Allen _{1,

2} , Jae Hyeon Park _{1,

2} , Steven J Jonas _{1,

6,

7,

8} , Nam-Joon Cho _{4,

5} , Paul S Weiss _{1,

2,

4,

9,

10}

Affiliation

California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
SKKU-UCLA-NTU Precision Biology Research Center, Sungkyunkwan University, Suwon 16419, Republic of Korea.
School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.
Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California 90095, United States.
Children's Discovery and Innovation Institute, University of California, Los Angeles, Los Angeles, California 90095, United States.
Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, California 90095, United States.
Department of Bioengineering, University of California, Los Angeles, Los Angeles, California 90095, United States.
Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States.

Supported lipid membranes are versatile biomimetic coatings for the chemical functionalization of inorganic surfaces. Developing simple and effective fabrication strategies to form supported lipid membranes with micropatterned geometries is a long-standing challenge. Herein, we demonstrate how the combination of chemical lift-off lithography (CLL) and easily prepared lipid bicelle nanostructures can yield micropatterned, supported lipid membranes on gold surfaces with high pattern resolution, conformal character, and biofunctionality. Using CLL, we functionalized gold surfaces with patterned arrays of hydrophilic and hydrophobic self-assembled monolayers (SAMs). Time-lapse fluorescence microscopy imaging revealed that lipid bicelles adsorbed preferentially onto the hydrophilic SAM regions, while there was negligible lipid adsorption onto the hydrophobic SAM regions. Functional receptors could be embedded within the lipid bicelles, which facilitated selective detection of receptor-ligand binding interactions in a model streptavidin-biotin system. Quartz crystal microbalance-dissipation measurements further identified that lipid bicelles adsorb irreversibly and remain intact on top of the hydrophilic SAM regions. Taken together, our findings indicate that lipid bicelles are useful lipid nanostructures for reproducibly assembling micropatterned, supported lipid membranes with precise pattern fidelity.

中文翻译：

使用化学剥离光刻技术进行脂质 Bicelle 微图案化。

支持的脂质膜是用于无机表面化学功能化的多功能仿生涂层。开发简单有效的制造策略来形成具有微图案几何形状的支撑脂质膜是一个长期存在的挑战。在此，我们展示了如何将化学剥离光刻（CLL）与易于制备的脂质双胶纳米结构相结合，在金表面上产生具有高图案分辨率、保形特性和生物功能的微图案化、支撑的脂质膜。使用 CLL，我们用亲水性和疏水性自组装单层 (SAM) 图案阵列对金表面进行功能化。延时荧光显微镜成像显示，脂质 bicelles 优先吸附到亲水性 SAM 区域，而疏水性 SAM 区域的脂质吸附可忽略不计。功能性受体可以嵌入脂质双分子内，这有助于选择性检测模型链霉亲和素-生物素系统中的受体-配体结合相互作用。石英晶体微天平耗散测量进一步确定脂质 bicelles 不可逆吸附并在亲水 SAM 区域顶部保持完整。总而言之，我们的研究结果表明，脂质 bicelles 是有用的脂质纳米结构，可用于以精确的图案保真度可重复地组装微图案化、支撑的脂质膜。

更新日期：2020-03-09

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