A long-term experiment in an anaerobic ammonium oxidation (anammox) reactor showed that anammox consortia could perform a stable and efficient Fe(II)-dependent dissimilatory nitrate reduction to ammonium (DNRA) coupled to the anammox (DNRA-anammox) process by controlling the EDTA-2Na/Fe(II) ratio and pH, with a total nitrogen removal rate (TNRR) of 0.23 ± 0.01 kg-N/m³/d. Anammox bacteria (Candidatus Kuenenia) were the dominant and functional microbes in such a nitrate wastewater treatment system. Visual MINTEQ analysis showed that the EDTA-2Na/Fe(II) molar ratio affected the influent composition of Fe and EDTA species and hence nitrate removal, while pH influenced both nitrate removal and the coupling degree of the Fe(II)-dependent DNRA-anammox process due to its own physiology. The kinetic simulation results showed that excess EDTA-2Na imposed a competitive inhibition on the Fe(II)-dependent DNRA-anammox process, and the Bell-shaped (A), (B), (C) and Ratkowsky models could be used to explore the pH dependency of the Fe(II)-dependent DNRA-anammox process.

在厌氧氨氧化（anammox）反应器中进行的长期实验表明，通过控制厌氧氨氧化（DNRA-anammox）过程，厌氧氨氧化联合体可以稳定高效地进行与厌氧氨氧化（DNRA-anammox）耦合的Fe（II）依赖性硝酸盐异化还原成铵（DNRA）过程。 EDTA-2Na/Fe(II)比值和pH值，总氮去除率(TNRR)为0.23 ± 0.01 kg-N/m ³ /d。厌氧氨氧化菌（ Candidatus Kuenenia）是该硝酸盐废水处理系统中的优势微生物和功能微生物。视觉 MINTEQ 分析表明，EDTA-2Na/Fe(II) 摩尔比影响 Fe 和 EDTA 物质的进水组成，从而影响硝酸盐去除，而 pH 影响硝酸盐去除和 Fe(II) 依赖性 DNRA- 的耦合程度。厌氧氨氧化过程由于其自身的生理学原因。动力学模拟结果表明，过量的EDTA-2Na对Fe(II)依赖性DNRA-厌氧氨氧化过程产生竞争性抑制，钟形(A)、(B)、(C)和Ratkowsky模型可用于探索 Fe(II) 依赖性 DNRA-anammox 过程的 pH 依赖性。